| 出芽酵母蛋白激酶TOS3的过量表达及其在热胁迫应答中的作用 |
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| 引用本文： | 钟彦,黄鹏,郄蓓蓓,刘立永,刘科.出芽酵母蛋白激酶TOS3的过量表达及其在热胁迫应答中的作用[J].四川大学学报(自然科学版),2009,46(6):1811-1816. |
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| 作者姓名： | 钟彦 黄鹏 郄蓓蓓 刘立永 刘科 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610064 |
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| 摘 要： | 出芽酵母SNF1蛋白激酶参与葡萄糖阻遏和细胞胁迫应答.TOS3可通过磷酸化激活SNF1,参与SNF1调控的信号途径.本研究利用PCR方法扩增tos3基因的蛋白质编码序列,克隆到多拷贝表达载体pYES2/NTA上构建真核表达载体,转化酵母细胞并诱导TOS3过量表达.带HIS6标签的重组TOS3蛋白通过免疫印迹得以鉴定.进一步研究了过量表达TOS3对细胞热胁迫耐受性的影响,发现在热胁迫处理条件下,TOS3过量表达可恢复△snf1突变体细胞的生长缺陷,表明TOS3可能通过不依赖SNF1的其他信号途径参与细胞对热胁迫应答的调控.
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| 关 键 词： | 出芽酵母 过量表达 热胁迫应答 |
Overexpression of protein kinase TOS3 and its role in heat stress response in budding yeast Saccharomyces cerevisiae |
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| ZHONG Yan,HUANG Peng,QIE Bei-Bei,LIU Li-Yong and LIU Ke.Overexpression of protein kinase TOS3 and its role in heat stress response in budding yeast Saccharomyces cerevisiae[J].Journal of Sichuan University (Natural Science Edition),2009,46(6):1811-1816. |
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| Authors: | ZHONG Yan HUANG Peng QIE Bei-Bei LIU Li-Yong and LIU Ke |
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| Institution: | Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University |
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| Abstract: | |
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| Keywords: | TOS3 TOS3 Saccharomyces cerevisiae overexpression heat stress response |
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