| 鹅掌楸碱的抗氧化性及稳定性研究 |
| |
| 引用本文： | 王天山,江洁滢,李自顺,张健秋,刘沛铭,陈永生.鹅掌楸碱的抗氧化性及稳定性研究[J].海南师院学报,2013(4):410-412. |
| |
| 作者姓名： | 王天山 江洁滢 李自顺 张健秋 刘沛铭 陈永生 |
| |
| 作者单位： | [1]海南师范大学海南省热带药用植物化学教育部重点实验室,海南海口571158 [2]海南师范大学化学与化工学院,海南海口571158 [3]海南华侨中学高中部,海南海口570206 |
| |
| 基金项目： | 海南省自然科学基金项目（213018）;海南师范大学2013年度“大学生创新创业训练计划”项目（2013116580） |
| |
| 摘 要： | 采用DPPH法评价鹅掌楸碱的抗氧化活性及在不同理化条件下的稳定性.方法：采用DPPH作为自由基体系,通过测定加入鹅掌楸碱后对DPPH溶液吸光度值的改变来计算出鹅掌楸碱对DPPH自由基的清除率和IC50值,以此评价鹅掌楸碱的抗氧化活性;另外,测定鹅掌楸碱受到光,热和酸碱介质处理前后鹩掌楸碱吸光度值的改变,以此评价鹅掌楸碱在这些理化条件下的稳定性.实验表明鹅掌揪碱对DPPH自由基的IC50值为0.70mg/mL.其在弱碱性介质中可稳定存在,5小时60℃恒温处理对鹅掌楸碱稳定性无明显影响,然而在酸性介质或者室内自然光照射会引起吸光度值的下降.鹅掌楸碱具有一定的抗氧化活性,在室温,避光和弱碱性介质下可稳定存在.
|
| 关 键 词： | 鹅掌楸碱 DPPH自由基 抗氧化活性 稳定性 |
Study on the Antioxidant Activity and Stability of Liriodenine |
| |
| WANG Tianshan,JIANG Jieying,LI Zishun,ZHANG Jianqiu,LIU Peiming,CHEN Yongsheng.Study on the Antioxidant Activity and Stability of Liriodenine[J].Journal of Hainan Normal University(Humanities and Social Sciences),2013(4):410-412. |
| |
| Authors: | WANG Tianshan JIANG Jieying LI Zishun ZHANG Jianqiu LIU Peiming CHEN Yongsheng |
| |
| Institution: | 1. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University ,Haikou 571158, China ; 2. College of Chemistry and Chemical Engineering, Hainan Normal University,Haikou 571158, China ; 3. Senior High Department of Hainan Overseas Chinese Middle School,Haikou 570206, China) |
| |
| Abstract: | To evaluate the antioxidant activity of Liriodenine by DPPH method, and the stability in different physio- chemical conditions, using DPPH as free radical system, the antioxidant activity of Liriodenine was evaluated by the clearance rate and the ICso value, which was calculated from the absorption value of residue DPPH after introducing Liriodenine to be antioxidant; and the stability of Liriodenine in different physiochemical conditions was evaluated on the different absorption value after treating by light, heat and acid-base medium. The result showed that the IC50 value of Liriodenine scavenging the DPPH free radical was 0.70 mg/mL. The stability experiments showed that Liriodenine is stable in weak alkaline medium and 60 ℃ heating treatment for 5 h. Liriodenine exhibits weak antioxidant activity with IC50= 0.70 mg/mL to DPPH free radical, and can exist stably in room temperature, avoiding light and weak alkaline medium. |
| |
| Keywords: | Liriodenine DPPH free radical antioxidant activity stability |
| 本文献已被 维普 等数据库收录！ |
|
