可降解Hr-BMP复合胶原膜修复腭部骨缺损的实验研究

目的：观察腭部扁平骨引导性骨再生现象（GBR），探讨利用GBR修复腭裂骨缺损的可能性。方法：建立幼犬腭部骨缺损实验动物模型，应用常规组织学检查术、免疫荧光显微法、X线及扫描电子显微术在实验过程的不同阶段进行观察研究。结果：1）借助复合人重组骨形成蛋白（Hr-BMP）胶原膜GBR，腭部骨缺损可以完全修复；2）胶原膜可提供成骨所需的密闭空间；3）复合Hr-BMP胶原膜具有良好的组织相容性和安全性；4）复合Hr-BMP胶原膜GBR所致骨形成的量及速度均高于其它对照。结论：1）复合Hr-BMP胶原GBR具有确实有效的骨引导和骨诱导性，在骨缺损修复的早期阶段即可产生大量的新骨；2）复合Hr-BMP胶原膜植入后4周内的成骨活动最活跃，胶原膜和复合Hr-BMP胶原膜在其降解吸收过程中，不干扰后续的成骨活动；3）仍有必要构建具有适合的吸收降 解性和一定力学强度的隔膜材料。

Studies on repairing of the palate bone defects of experimental animal with collagen membrane composite of human recombinant bone morphogenetic protein

Aim: To observe the guided bone regeneration(GBR) in the tabular bone of palate and explore the possibility of repairing of bone defects in cleft palate by GBR. Methods: Puppies were used to build the model of palate bone defects. The experimental process was checked by general histological examination, fluorescence microscopy, X-ray and scanning electron microscopy at different time. Results: 1. The palate bone defects can be repaired completely by GBR with collagen composite of human recombinant morphogenetic protein (Hr-BMP). 2. The collagen membrane can provide the sealed chamber for osteogenetic process. 3. collagen membrane incorporating with Hr-BMP have a good histological compatibility and safety. 4. The volume and speed of osteogenesis by GBR with Hr-BMP was much more higher than that of the other groups. Conclusion: 1. Collagen membrane incorporated Hr-BMP has the effectiveness of bone guided and bone induced, which can prompt the formation of a great deal of new bone in bone defect in early time of repairing. 2. The activity of new bone formation is the highest in four weeks after collagen membrane/Hr-BMP implantation. During absorbing period, both collagen membrane and collagen membrane/Hr-BMP did not disturb the process of bone formation. 3. Designing and constructing suitable membranes which should posses the properties of appropriate absorbable and degradable time, fixed dynamic strength for succeeding in GBR is still necessary.