蛛丝蛋白载药膜的结构表征及其降解性能研究

为治疗化疗后的口腔黏膜炎，以敬钊缨毛蛛的天然蛛丝作为成膜材料制备载药膜剂，并对膜剂特性进行表征。采用溶剂浇铸法制备出蛛丝蛋白膜，通过水接触角实验、傅立叶红外光谱法、X-射线衍射法、扫描电镜评价膜剂的结构特征，探讨了膜剂的降解性。本试验所制备膜剂表征实验证明药物成功载入膜中，降解实验表明膜剂在人工唾液中7 d内的降解失重率仅5%，在人工胃液中72 h降解失重率达到65%以上。以天然蛛丝-六氟异丙醇溶液为成膜液，采用溶剂浇铸法可成功制备出载有肌苷药物的口腔膜剂。

DEGRADATION PROPERTY AND STRUCTURAL CHARACTERIZATION OF NATURAL SPIDER SILK PROTEIN DRUG-LOADED FILM

To treat oral mucosal inflammation after chemotherapy, the natural silk of the Chilobrachys guangxiensis is used as a film-forming material to prepare a drug loaded film, and the characteristics of the film are characterized. Spider silk protein matrix film was designed and prepared by solvent casting method, and inosine was successfully loaded on the film. The structural characteristics of the film were evaluated by the contact angle test, fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy, and the degradability of the film was explored. A series of structural characterization experiments of the prepared film proved that the drugs were successfully loaded on the film; the degradation experiment showed that the weight loss rate of the film in artificial saliva was only 5% within 7 days, while in artificial gastric juice was more than 65% after 72 hours. Oral film with inosine can be prepared successfully by natural spider silk-hexafluoroisopropyl alcohol solution as film-forming solution by solvent casting.