| 聚(NIPAAM-EDMA)整体柱的制备及其对尿样中氢化可的松的富集分析 |
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| 引用本文： | 贾艳花,刘海燕,杨宁,段艳慧,杨更亮. 聚(NIPAAM-EDMA)整体柱的制备及其对尿样中氢化可的松的富集分析[J]. 河北大学学报(自然科学版), 2012, 32(2): 154-158 |
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| 作者姓名： | 贾艳花 刘海燕 杨宁 段艳慧 杨更亮 |
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| 作者单位： | 河北大学药学院,河北保定,071002 |
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| 基金项目： | 国家自然科学基金资助项目,河北省科技厅资助项目,河北省教育厅资助项目,科技部重大新药创制科技重大专项十二五计划第二批课题,河北大学自然科学基金资助项目 |
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| 摘 要： | 以N-异丙基丙烯酰胺(NIPAAM)为单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备了一种能有效去除尿样中的大分子物质,并能同时富集尿样中氢化可的松的整体柱.实验结果表明:氢化可的松在5～30 mg/L线性关系良好(R=0.999 3,n=6),检测限为0.012 mg/L;绝对回收率79％～83％;日内、日间精密度均＜5.0％,整体柱能够很好地富集尿样中的氢化可的松,并且能很好地除去尿样中的大分子物质及杂质,能满足分析的要求.
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| 关 键 词： | N-异丙基丙烯酰胺 整体柱 富集分析 氢化可的松 |
Preparation of poly(NIPAAM-EDMA) monolithic column for enriching and analyzing hydrocortisone from urine |
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| JIA Yan-hua , LIU Hai-yan , YANG Ning , DUAN Yan-hui , YANG Geng-liang. Preparation of poly(NIPAAM-EDMA) monolithic column for enriching and analyzing hydrocortisone from urine[J]. Journal of Hebei University (Natural Science Edition), 2012, 32(2): 154-158 |
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| Authors: | JIA Yan-hua LIU Hai-yan YANG Ning DUAN Yan-hui YANG Geng-liang |
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| Affiliation: | (College of Pharmacy,Hebei University,Baoding 071002,China) |
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| Abstract: | A new monolithic column have been prepared which was composed of copolymer that the N-isopropylacrylamide(N-IPAAM) was the monomer and the ethyleneglycoldimethacrylate(EDMA) was the cross-linker.The monolith was used for removing the macromoleculer and enriching the hydrocortisone in urine sample.The result showed that hydrocortisone had a good linear(R=0.999 3,n=6) when the concentration of the hydrocortisone in the range of 5-30 mg/L,the limit of detection(LOD) was 0.012 mg/L.The absolutely recovery was 79%-83%.The inter-day RSD and intra-day RSD was less than 5%.This method is conforming to analysis. |
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| Keywords: | N-isopropylacrylamide monolithic column enrichment analysis hydrocortisone |
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