刘氏短须螨危害对水杉生理生化物质及挥发物的影响

利用植物生理和生化方法测定了水杉被刘氏短须螨危害前后针叶内叶绿素、可溶性糖、游离氨基酸、黄酮、单宁、挥发物和保护酶活性的变化。结果表明:水杉叶片被刘氏短须螨危害后,叶绿素含量降低18.5%,可溶性糖含量降低12.8%,游离氨基酸含量降低12.2%,黄酮含量升高26.6%,单宁含量升高25.8%,过氧化物酶和超氧化物歧化酶活性分别升高65%和139%。刘氏短须螨侵染后,水杉叶片挥发物组分变化不大,但各组分构成比例发生较明显变化。挥发物主要成分由萜类、萜类氧化物、酯类、烃类、杂环类及少量的其他物质组成,健康叶片共检测到35种物质,被害叶片检测到33种,两者共有物质28种。研究结果说明,刘氏短须螨的刺吸危害引起了寄主植物明显的生理生化变化。

Effects of Brevipalpus lewisi infested on physiological, biochemical and volatile components in Metasequoia glyptostroboides leaves

Chlorophyll,soluble sugar,free amino acids,flavonoids,tannin,volatiles components and the activities of protective enzymes in healthy and mite-infested leaves of Metasequoia glyptostroboides were measured by plant physiological and biochemistry methods.The results showed that compared with healthy leaves of M.glyptostroboides,the content of chlorophyll,soluble sugar and free amino acids reduced 18.5 %,12.8 % and 12.2 % respectively,while the content of flavonoids and tannin increased 26.6 % and 25.8 %,respectively.The activities of peroxidase increased 65 %,and the activities of superoxide dismutase increased 139 %,which were obviously higher than that of control.35 compositions in healthy leaves and 33 compositions in infested leaves were detected and identified respectively by SPME-GC-MS.28 compositions of which were the same.The results suggested that some physiological and biochemical parameters were changed in M.glyptostroboides leaves injured by Brevipalpus lewisi.