| 短小芽孢杆菌JK-SX001非蛋白抗菌物质研究 |
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| 引用本文： | 颜爱勤,吴小芹,叶建仁,凡波. 短小芽孢杆菌JK-SX001非蛋白抗菌物质研究[J]. 南京林业大学学报(自然科学版), 2012, 36(3): 13-16 |
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| 作者姓名： | 颜爱勤 吴小芹 叶建仁 凡波 |
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| 作者单位： | 南京林业大学,江苏省有害生物入侵预防与控制重点实验室, 江苏 南京210037 |
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| 基金项目： | 国家林业公益性行业科研专项项目，江苏省高校优势学科建设工程资助项目 |
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| 摘 要： | 为探讨短小芽孢杆菌JK-SX001菌株非蛋白抗菌物质对杨树溃疡病的作用机制,研究了该菌株非蛋白抗菌物质的提取方法及抗菌物质对金黄壳囊孢(Cytospora chrysosperma)、拟茎点霉(Phomopsis macrospora)和七叶树壳梭孢(Fusicoccum aesculi)真菌菌丝及分生孢子的抑制机制,同时对其部分理化性质进行了测定。结果表明,JK-SX001菌株产生的非蛋白抗菌物质能强烈抑制病原真菌菌丝生长和分生孢子萌发,且能使分生孢子溶解。光照、温度对抗菌物质影响较小,而有效存储期对抗菌物质影响相对较大。
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| 关 键 词： | 短小芽孢杆菌 非蛋白抗菌物质 拮抗机制 |
Isolation, antifungal effect and characteristics of non-protein substances produced by Bacillus pumilus JK-SX001 |
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| YAN Aiqin , Wu Xiaoqin , YE Jianren , FAN Bo. Isolation, antifungal effect and characteristics of non-protein substances produced by Bacillus pumilus JK-SX001[J]. Journal of Nanjing Forestry University(Natural Sciences ), 2012, 36(3): 13-16 |
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| Authors: | YAN Aiqin Wu Xiaoqin YE Jianren FAN Bo |
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| Affiliation: | (Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing Forestry University,Nanjing 210037,China) |
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| Abstract: | In order to explore the effect of non-protein antifungal substances produced by Bacillus pumilus strain JK-SX001 and its mechanisms on poplar canker disease,three pathogenic fungi species Cytospora chrysosperma,Phomopsis macrospora and Fusicoccum aesculi were used.The extraction methods of the non-protein antifungal substances and its inhibitory mechanisms on mycelia and germinated-spores were studied,while we also measured some physical and chemical properties of the non-protein antifungal substances.The results showed that the non-protein antifungal substances had a strong inhibition to the mycelia growth and spore germination,and could dissolve the spore.Valid storage life had great influence on the substances.However,illumination and temperature had little effect on the activity of the antifungal substances. |
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| Keywords: | Bacillus pumilus non-protein antifungal substances antagonistic mechanism |
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