| 利用两种方法筛选拟南芥uro突变体的回复子 |
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| 引用本文： | 张亮,杨扬,孙越,徐艳飞,俞琛,周薇,唐洪波,李小方.利用两种方法筛选拟南芥uro突变体的回复子[J].华东师范大学学报(自然科学版),2010,2010(4):85-91. |
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| 作者姓名： | 张亮 杨扬 孙越 徐艳飞 俞琛 周薇 唐洪波 李小方 |
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| 作者单位： | 华东师范大学,生命科学学院,上海,200062 |
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| 基金项目： | 863项目，国家自然科学基金面上项目 |
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| 摘 要： | 拟南芥URO基因与生长素的代谢密切相关,为了深入了解URO基因的作用机制及其所参与的遗传途径,以拟南芥叶发育异常的半显性突变体uro(upright rosette)为材料,利用EMS诱变和Ac/Ds转座子系统这两种方法筛选uro突变体的回复子.通过这两种方法获得了一些回复子,并对这些回复子进行了验证和初步分析,为今后的研究工作奠定了良好的基础.
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| 关 键 词： | 拟南芥 URO EMS诱变 Ac/Ds转座子系统 拟南芥 URO EMS诱变 Ac/Ds转座子系统 |
| 收稿时间： | 2009-4-1 |
| 修稿时间： | 2009-8-1 |
Screening the suppressors of uro by EMS mutagenesis and Ac/Ds transposon system |
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| ZHANG Liang,YANG Yang,SUN Yue,XU Yan-fei,YU Chen,ZHOU Wei,TANG Hong-bo,LI Xiao-fang.Screening the suppressors of uro by EMS mutagenesis and Ac/Ds transposon system[J].Journal of East China Normal University(Natural Science),2010,2010(4):85-91. |
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| Authors: | ZHANG Liang YANG Yang SUN Yue XU Yan-fei YU Chen ZHOU Wei TANG Hong-bo LI Xiao-fang |
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| Institution: | School of Life Science, East China Normal University, Shanghai 200062, China |
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| Abstract: | It was suggested that URO function might be associated with auxin mediated plant development. In order to know the role and the genetic net work ofURO gene, suppressors(double mutants with suppressed phenotype of uromutant) were screened through EMS mutagenesis and Ac /Ds transposon system. Several suppressors have been selected, validated and further analyzed. These suppressors will provide important clews for the function network of URO gene. |
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| Keywords: | URO |
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