| 首蓿(Medicago sative L.)体胚发生及成苗的研究——Ⅰ.增加健康体胚数量及提高其成苗率 |
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| 引用本文： | 孙景欣,傅家瑞,黄学林,黄上志.首蓿(Medicago sative L.)体胚发生及成苗的研究——Ⅰ.增加健康体胚数量及提高其成苗率[J].中山大学学报(自然科学版),1991(2). |
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| 作者姓名： | 孙景欣 傅家瑞 黄学林 黄上志 |
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| 作者单位： | 中山大学生物学系，中山大学生物学系，中山大学生物学系，中山大学生物学系 |
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| 基金项目： | 国家‘863’高技术计划资助项目 |
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| 摘 要： | 盆栽苜蓿第三叶柄和无菌苗下胚轴在附加2,4-D及KT的B_5h固体培养基上均能诱导出较多胚性愈伤组织,再经附加2,4-D的B_5g液体培养基诱导,在加入L-脯氨酸、(NH_4)_2SO_4及KNO_3的液体培养基中摇床培养,则形成大量比较健康的体胚。用这类体胚接种于不含任何激素,但附加维生素C和干酶母粉,并用2%麦芽糖代替3%蔗糖的1/2SH固体培养基上,20天后,96%体胚长成完整的小苗。这些小苗移至盆栽,移植后14天,成活率达80%。
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| 关 键 词： | 苜蓿 体胚发生 转化 |
The Study on the Somatic Embryogenesis and Plantlet Formation of Medicago sative L.1.Increase of healthy somatic embryos and plantlet formation |
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| Sun Jingxin,Fu Jiarui,Huang Xuelin,Huang Shangzhi.The Study on the Somatic Embryogenesis and Plantlet Formation of Medicago sative L.1.Increase of healthy somatic embryos and plantlet formation[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1991(2). |
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| Authors: | Sun Jingxin Fu Jiarui Huang Xuelin Huang Shangzhi |
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| Institution: | Sun Jingxin;Fu Jiarui;Huang Xuelin;Huang Shangzhi Department of Biology |
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| Abstract: | Abundant embryogenic callus were induced when the third petioles of alfalfa
plants grown in soil or hypocotyls of sterile alfalfa seedlings were cultured on
solid B_5h medium with 2,4-D and KT.The callus were induced to form somatic
embryos in liguid B_5g medium with 2,4-D.After subcultured in liguid B_5g medium
with L-proline,(NH_4)_2SO_4 and KNO_3,a large number of healthy somatic embryos
were obtained.Somatic embryos were cultured on solid 1/2 SH medium containing
vitamin c and yeast extract with 2% maltose(substitued for 3% sucrose)and wi-
thout addition hormone after ineubating for 20 days,96% of them developed
into plantlets.80% plantlets survived after transplanting to soil for 14 days. |
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| Keywords: | Medicago satire L somatie embryogenesis transfer |
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