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| 大白菜籽苗的苗龄及生长条件对子叶离体培养不定芽再生的影响 | |
| 引用本文： | 王火旭,王关林,方宏筠,魏毓棠,宋维慧. 大白菜籽苗的苗龄及生长条件对子叶离体培养不定芽再生的影响[J]. 辽宁师范大学学报(自然科学版), 2000, 23(2): 163-165 |
| 作者姓名： | 王火旭 王关林 方宏筠 魏毓棠 宋维慧 |
| 作者单位： | 1. 辽宁师范大学,生物工程研究所,辽宁,大连,116029 2. 沈阳农业大学,辽宁,沈阳,110161 3. 辽宁省对外贸易公司,辽宁,大连,116011 |
| 基金项目： | 国家自然科学基金资助项目!( 3 95 70 3 81) |
| 摘 要： | 以大白菜自交系ＡＢ－８１为试材，对籽苗的苗龄以及生长条件如光照，播种基本培养基及蔗糖浓度等外界因子对子叶离体培养不定芽再生的影响进行了研究。３￣４ｄ苗龄和子叶不定芽再生能力最强；０￣２％的蔗糖浓度优于４％￣６％的蔗糖浓度，播种的基本培养基为１／４ＭＳ对子叶的不定芽再生最为有利，籽苗在２０００ｌｘ左右的日光下比在２９００ｌｘ或１２００ｌｘ荧光下培养更利于子叶的不定芽再生。 |
| 关 键 词： | 大白菜 子叶 离体培养 苗龄 生长条件 籽苗 |
| 修稿时间： | 1999-08-26 |
Effects of Seedling Age and Growth Conditions on in vitro Shoot Regeneration from Cotyledons of Brassica campestris ssp.pekinensis |
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| WANG Huo-xu,WANG Guan-lin,FANG Hong-jun,WEI Yu-tang,SONG Wei-hui. Effects of Seedling Age and Growth Conditions on in vitro Shoot Regeneration from Cotyledons of Brassica campestris ssp.pekinensis[J]. Journal of Liaoning Normal University(Natural Science Edition), 2000, 23(2): 163-165 | |
| Authors: | WANG Huo-xu WANG Guan-lin FANG Hong-jun WEI Yu-tang SONG Wei-hui |
| Abstract: | The self-bred line AB-81 of Brassica campestris ssp.pekinensis was used in the study of the effects of seedling age and growth conditions on in vitro shoot regeneration from cotyledons.The cotyledons from 3~4 day seedlings ha d the highest adventitious shoot regeneration competence.The sucrose content at 0~2% in the seed germination medium was better than that at 4%~6%.Among the te st ed basal media for seed germination,1/4 MS was the optimum.The sunlight of 20 0 00 lx during seedling growth was more advantageous to in vitro shoot regener ation than the fluorescent light of 2 900 lx or 1 200 lx. |
| Keywords: | Brassica campestris ssp pekinensis cotyledons in vitro shoot reg eneration seedling age growth conditions |
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