| 绿脓杆菌外毒素A与Ⅱ型单纯疱疹病毒糖蛋白嵌合表达质粒的构建及其免疫原性的研究 |
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| 引用本文： | 马超 黄思扬 蒋琳 杨福军 王革 辛芳. 绿脓杆菌外毒素A与Ⅱ型单纯疱疹病毒糖蛋白嵌合表达质粒的构建及其免疫原性的研究[J]. 兰州大学学报(自然科学版), 2005, 41(5): 48-51 |
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| 作者姓名： | 马超 黄思扬 蒋琳 杨福军 王革 辛芳 |
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| 作者单位： | [1]兰州生物制品研究所,甘肃兰州730046 [2]兰州大学生命科学学院,甘肃兰州730000 |
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| 摘 要： | 构建外毒素结构域Ⅰ a基因(EPA103)与Ⅱ型单纯疱疹病毒糖蛋白(HSV-2-gD)嵌合蛋白表达载体(pET-EgD),得到嵌合表达蛋白.利用外毒素结构域Ⅰ a基因表达蛋白的佐剂效应,以pET-EgD表达嵌合蛋白免疫Balb/c小鼠.结果显示:嵌合表达蛋白(pET-EgD)免疫小鼠,可在小鼠体内诱导产生抗HSV-2特异性抗体.嵌合表达蛋白(pET-EgD)具有较强的免疫原性,为开发研究Ⅱ型单纯疱疹核酸疫苗奠定了基础.
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| 关 键 词： | 绿脓杆菌外毒素A 结构域Ⅰa 嵌合表达 |
| 文章编号： | 0455-2059（2005）05-0048-04 |
| 收稿时间： | 2005-04-28 |
| 修稿时间： | 2005-04-28 |
Study of the chimeras of Pseudomonas aeruginosa exotoxin A and HSV-2-gD and its immunogenicity |
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| MA Chao, HUANG Si-yang, JIANG Lin, YANG Fu-jun, WANG Ge, XIN Fang. Study of the chimeras of Pseudomonas aeruginosa exotoxin A and HSV-2-gD and its immunogenicity[J]. Journal of Lanzhou University(Natural Science), 2005, 41(5): 48-51 |
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| Authors: | MA Chao HUANG Si-yang JIANG Lin YANG Fu-jun WANG Ge XIN Fang |
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| Affiliation: | 1. Lanzhou Institute of Biological Products, Lanzhou, 730046, China; 2. School of Life Sciences, Lanzhou University, Lanzhou, 730000, China |
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| Abstract: | The chimeras were constructed with pET-EPA103 and HSV-2-gD by using the molecular cloning technique and the chimeras protein pET-EgD was used to immune Balb/c mice. The results showed that chimeras protein could stimulate mice to produce anti-HSV-2 specific antibody. All of these showed that pET-EgD has the function of immunogenicity and this would provide the basis for research into the molecular adjuvant of nucleic acid vaccine. |
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| Keywords: | Pseudomonas aeruginosa exotoxin A Domain Ia chimeras |
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