双特异性酪氨酸磷酸化调节激酶在宫颈病变组织和癌细胞中的表达

目的探讨双特异性酪氨酸磷酸化调节激酶1b(DYRK1b)在宫颈病变组织和癌细胞中的表达情况及其作用.方法选取宫颈癌患者127例为研究组,同期确诊为慢性宫颈炎患者32例为对照组,收集上述患者石蜡组织标本,免疫组化SP法检测DYRK1b蛋白表达情况.依据宫颈癌细胞系He La 229和Si Ha细胞将标本分为实验组(加DYRK1b抑制剂Az191)、对照组(不加Az191),Western blod法检测细胞中DYRK1蛋白表达情况,MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡情况.结果子宫颈鳞癌阳性表达率明显高于慢性宫颈炎、低级别鳞状上皮内病变、高级别鳞状上皮内病变,差异均具有统计学意义(P0.01);高级别鳞状上皮内病变明显高于慢性宫颈炎、低级别鳞状上皮内病变,差异具有统计学意义(P0.05).在He La 229和Si Ha细胞中,DYRK1b蛋白表达量随Az191剂量增加而减少,且均明显低于对照组,差异具有统计学意义(P0.01).研究组He La 229和Si Ha细胞抑制率随Az191浓度增加而提升,均明显高于对照组,差异具有统计学意义(P0.05).10μmol/L的Az191作用后,研究组He La 229和Si Ha细胞凋亡率明显高于对照组,差异具有统计学意义(P0.05).结论宫颈病变过程中DYRK1b蛋白表达水平逐渐提升,宫颈癌组织和细胞中均呈高表达;下调DYRK1b蛋白表达后可抑制宫颈癌细胞增殖,促进凋亡.

Expression of Dual Specificity Tyrosine Phosphoralation-Regulated Kinase in Cervical Lesions and Cancer Cells

ObjectiveTo investigate the expression and its effects of dual specificity tyrosine phosphorylation-regulated kinase 1b (DYRK1b) in cervical lesions and cancer cells.Method127 cervical cancer patients including 38 cases of cervical squamous cell carcinoma,68 cases of low-grade squamous intraepithelial lesions,and 21 cases of high grade squamous intraepithelial lesions;32 cases of patients diagnosed with chronic cervicitis were selected as the controls;the patients' paraffin-embedded tissue samples were prepared and DYRK1b protein expressions in the samples were detected by immunohistochemical SP method.Cervical cancer cell line HeLa 229 and SiHa cells were divided into experimental group (incubated with DYRK1b inhibitor Az191) and control group (incubated without Az191);the expression of DYRK1 protein was detected by Western blot,the cell proliferation by MTT,and the cell apoptosis by flow cytometry.ResultsThe positive expression rate of cervical squamous cell carcinoma was significantly higher than that of chronic cervicitis,low grade squamous intraepithelial lesion and high grade squamous intraepithelial lesions,and the difference was statistically significant (P<0.01);that in high grade squamous intraepithelial lesion was significantly higher than that in chronic cervicitis and low grade squamous intraepithelial lesions,with statistically significant differences (P<0.05).DYRK1b protein expressions in HeLa 229 and SiHa cells decreased with the increase of Az191 dosage,and were significantly lower than those in the control group,and the difference was statistically significant (P<0.01).The inhibition rate of HeLa 229 and SiHa in the study group increased with the increase of Az191 concentration,which was significantly higher than that in the control group (P<0.05).After the treatment with 10 μmol/L Az191,the apoptosis rate of HeLa 229,SiHa cell in the study group was significantly higher than that of the control group (P<0.05).ConclusionThe expression level of DYRK1b protein in the process of cervical lesions was gradually increased,and the cervical cancer tissues and cells showed a high expression of DYRK1b protein.Down-regulating the expression of DYRK1b may inhibit the proliferation of cervical cancer cells and promote the apoptosis of them.