银屑病TNF-α及c-myc表达的变化及意义

目的 探讨银屑病的免疫机制,为银屑病的临床诊治及新药研制提供实验依据.方法 利用细胞培养技术培养HaCaT细胞,采用ELISA法检测银屑病外周血TNF-α及c-myc的表达情况以及TNF-α刺激后c-myc在银屑病HaCaT细胞中的表达情况.结果 与正常对照组比较,银屑病组外周血血清中TNF-α及c-myc的水平明显升高(P<0.01);80 ng/mL的TNF-α可刺激HaCaT细胞高分泌c-myc蛋白,组间比较差异具有统计学意义(P<0.01).结论 TNF-α是通过影响c-myc的分泌在银屑病发生、发展过程中起重要作用的.

Change and Significance of Expression of TNF-alpha and c-myc in Psoriasis

Objective To study the immune mechanism of psoriasis and provide basis for clinical diagnosis and treatment of psoriasis and new drug research. Method Using cell culture technology to train HaCaT cells,and then the expression of TNF-alpha and c-myc in peripheral blood of psoriasis and the expression of the c-myc inthe HaCaT cells of psoriasis were detected by ELISA. Results Compared with normal control group,psoriasis group in peripheral blood serum TNF-alpha and c-myc level increased significantly (P<0 . 01 );The concentration of 80 ng/mL high TNF-alpha can stimulate HaCaT cells secrete c-myc protein,comparison between groups, significant difference (P<0 . 01 ). Conclusion TNF-alpha plays an important role in the process of development by influencing the c-myc secretion in patients with psoriasis,but the specific mechanism remains to be further research.