血液灌流吸附对犬内毒素血症模型的实验观察

目的本实验运用内毒素吸附柱,对比格犬内毒素血症模型进行灌流吸附作用,探讨其在犬体内吸附内毒素的有效性和安全性。方法静脉输注LPS(内毒素)复制犬内毒素血症模型,随机分为治疗组和对照组。治疗组采用血液灌流机配合内毒素吸附柱进行灌流吸附治疗,对照组采用不含内毒素吸附剂的空柱。分别选取多个时间点,采血测定犬相关的生理生化指标和内毒素含量。结果安全性检测:治疗组犬的体温持续下降,对照组犬的体温先升后降。与对照组比较,治疗组犬体温在灌流吸附90 min、120 min时,有显著性差异(P<0.05)。与对照组相比,治疗组犬在心率、血常规、血清总蛋白和血清白蛋白、电解质含量、凝血酶原时间、部分活化凝血酶原时间、血清补体C3浓度及纤维蛋白酶含量等生理指标,均未见显著性差异(P>0.05)。吸附效率检测:测定内毒素过滤柱灌流吸附60 min、90 min1、20 min三个时间段内毒素含量,治疗组犬血清中内毒素降低的程度与对照组相比,具有极显著差异(P<0.01)。结论内毒素吸附柱具有较好的血液相容性,具有吸附血清中内毒素的作用,可降低血清中内毒素水平。

Effect of Hemoperfusion Adsorption in Dog Model of Endotoxemia

Objective To explore the efficacy and safety of hemoperfusion adsorption in dogs.Methods Beagle dog model of endotoxemia was established by intravenous infusion of endotoxin LPS,and was randomly divided into treatment group and control group respectively.Treatment group was given the perfusion adsorption therapy using hemoperfusion machine and endotoxin adsorption column,and the control group was treated with the non-endotoxin adsorbent column.Blood physiological and biochemical parameters and endotoxin content were measured at multiple time points.Results Safety tests: body temperature dropped continuously in the treated dogs,but rose at first and then fell in the control dogs.In 90 min and 120 min after perfusion,body temperature was significantly different between the control and treatment group(P<0.05).Compared with the control group,there were no significant differences in heart rate,blood routine,serum total protein and albumin,electrolyte concentration, prothrombin time,activated partial prothrombin time,serum complement C3 concentration,thrombin content,and so forth(P>0.05).Detection of adsorption efficiency: the level of serum endotoxin was decreased significantly in the treatment group(P<0.01).Conclusion Serum endotoxin can be adsorbed and the level of serum endotoxin can be reduced by the endotoxin adsorption column,which is of good blood compatibility.