| 钙调素通过HSP70调控热激转录因子的活性 |
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| 引用本文： | 李冰,穆睿聆,李国良,孙大业,周人纲. 钙调素通过HSP70调控热激转录因子的活性[J]. 河北师范大学学报(自然科学版), 2004, 28(4): 401-406 |
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| 作者姓名： | 李冰 穆睿聆 李国良 孙大业 周人纲 |
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| 作者单位： | 河北省农林科学院,遗传生理研究所,河北,石家庄,050051;河北师范大学,生命科学学院,河北,石家庄,050016;河北师范大学,生命科学学院,河北,石家庄,050016;河北省农林科学院,遗传生理研究所,河北,石家庄,050051 |
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| 基金项目： | 国家重点基础研究专项经费资助项目(G1999011700)，国家自然科学基金资助项目(30270796)，河北省自然科学基金资助项目(301447) |
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| 摘 要： | 凝胶阻滞分析结果显示,70kD的热激蛋白(HSP70)抑制热激转录因子(HSF)的DNA结合活性;利用免疫共沉淀法在细胞提取液中检测到了CaMHSP70复合物的存在,并且热激后CaMHSP70复合物含量增加.表明钙调素(CaM)调控HSF活性可能是通过与HSP70结合起作用.
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| 关 键 词： | 钙调素 HSP70 热激转录因子 |
| 文章编号： | 1000-5854(2004)04-0401-06 |
| 修稿时间： | 2004-02-12 |
Calmodulin Regulates Activity of HSF Through HSP70 |
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| LI Bing. Calmodulin Regulates Activity of HSF Through HSP70[J]. Journal of Hebei Normal University, 2004, 28(4): 401-406 |
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| Authors: | LI Bing |
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| Abstract: | With gel mobility shift assay,it was found that DNA-binding activity of HSF was repressed by HSP70.It was also found that the CaM-HSP70 complex was existed in cytoplasmic extract by co-immunoprecipetation experiment,and concentration of CaM-HSP70 complex increased after heat shock.The results provide proofs for regulation of CaM on activity of HSF likely through binding to HSP70.Maize was used as experimental material. |
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