| Anti-H IV-1 Activities of 4 Telomerase Restrictors |
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| 作者姓名： | YU Xin WANG Jinghui de Giuli Morghen C Radaelli A Zanotto C Beggio p |
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| 作者单位： | [1]College of Pharmacy, Wuhan University, Wuhan 430072, Hubei, China [2]Medical School, Huangshi Institute of Technology, Huangshi 435003, Hubei, China [3]Laboratory of Molecular Virology, Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy |
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| 基金项目： | Supported by China Scholarship Counsil（CSC） and Wuhan Chenguang Program of Wuhan City （2003002016-24） |
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| 摘 要： | MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors（TRs） with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV（Human immtmodeficiency Virus） antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC （Peripheral Blood mononuclear cells） and CEM/GFP if the concentration of TRs was at 50μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.
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| 关 键 词： | 端粒末端转移酶 节气门 HIV 基因表达 |
| 文章编号： | 1007-1202（2007）06-1113-05 |
| 收稿时间： | 20 April 2007 |
| 修稿时间： | 2007-04-20 |
Anti-HIV-1 activities of 4 Telomerase Restrictors |
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| YU Xin WANG Jinghui de Giuli Morghen C Radaelli A Zanotto C Beggio p.Anti-H IV-1 Activities of 4 Telomerase Restrictors[J].Wuhan University Journal of Natural Sciences,2007,12(6):1113-1117. |
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| Authors: | Yu Xin Wang Jinghui Giuli Morghen C de Radaelli A Zanotto C Beggio P |
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| Institution: | (1) College of Pharmacy, Wuhan University, Wuhan, 430072, Hubei, China;(2) Medical School, Huangshi Institute of Technology, Huangshi, 435003, Hubei, China;(3) Laboratory of Molecular Virology, Department of Medical Pharmacology, University of Milan, Via Vanvitelli 32, 20129 Milano, Italy |
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| Abstract: | MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors(TRs) with minimum toxicity to
the selected cells. FACSort flow cytometer and Innotest P24 HIV(Human immunodeficiency Virus) antigen mAb ELISA Kit were used
to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC (Peripheral
Blood mononuclear cells) and CEM/GFP if the concentration of TRs was at 50 μmol/L or below, and the supernatant from PBMC
pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity,
which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.
Biography: YU Xin(1969–), male, Associate professor, Ph.D., present working at Huangshi Institute of Technology, research direction:
bioanalytical chemistry and biopharmacy. |
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| Keywords: | telomerase telomerase restrictor HIV anti-HIV Agents Screening |
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