单增李斯特菌对小鼠滋养层细胞炎性体的激活

为探讨单增李斯特菌(Lisetria monocytogenes,LM)诱导小鼠滋养层细胞炎性体激活在其致小鼠流产中的作用,在体外用LM处理小鼠滋养层细胞,观察LM在细胞内的分布,检测培养基中白细胞介素(interleukin,IL)-1β水平和细胞裂解液中天冬氨酸蛋白水解酶1(cysteinyl aspartate ...

Activiation of the inflammasomes in trophoblastic cells by Lisetria monocytogenes

To explore the role of Lisetria monocytogenes(LM)-induced mouse trophoblastic cell inflammasome activation in LM-induced mouse abortion, mouse trophoblastic cells were infected with LM. After the mouse trophoblastic cells were infected with LM in vitro,distribution of LM were observed. Interleukin(IL)-1β level in cell medium, cleavaged cysteinyl aspartate specific proteinase 1(caspase 1)level in the cell lysate and the amount of lactate dehydrogenase(LDH)release were detected. Results showed that LM distributed around the cell nucleus. Both IL-1β level in cell medium, cleavaged caspase 1 level in the cell lysate and the amount of LDH release were increased significantly in LM-infected group compared to control group, while the IL-1β level and the cleavaged caspase 1 level of the listeriolysin O(LLO)-deficient LM(Δ hly LM)-infected group were obviously lower than that of wild LM-infected group. Therefore, it is speculated that LM can activiate mouse trophoblastic inflammasome by LLO and promote the secretion of IL-1β and the pyrotosis of trophoblastic cells, which induced the mouse abortion. These results can provide a scientific basis for the clinical prevention and treatment on LM-induced abortion.