| HPLC内标法与化学法测定蜂蜜中糖含量的比较 |
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| 引用本文： | 刘伟,郭华.HPLC内标法与化学法测定蜂蜜中糖含量的比较[J].应用科技,2001,28(8):55-56,59. |
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| 作者姓名： | 刘伟 郭华 |
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| 作者单位： | 1. 哈慈集团儿童药业有限公司,黑龙江缓化 152000
2. 哈尔滨商业大学食品工程系,黑龙江哈尔滨 150076 |
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| 摘 要： | 蜂蜜中含有多种上糖类，采用化学法测定时间长，而且对蔗糖、葡萄糖成分的测定可能偏高于实际含量。采用液相色谱法，蜂蜜样品经水解后，以鼠李糖作为内标，差示折乐检测器检测，直接分离和测定蜂蜜中果糖、葡萄糖、蔗糖、麦芽糖含量。该方法快速、灵敏、准确、操作方便。
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| 关 键 词： | 高效液相色谱法 内标法 果糖 葡萄糖 蔗糖 麦芽糖 鼠李糖 蜂蜜 糖含量 方法比较 |
| 文章编号： | 1009-671(2001)08-0055-03 |
A Comparison between Methods for Detecting the Content of Carbohydrate with Honey of HPLC by Using Internal Standard Method and Chemiscal Method |
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| WU Yan-Hua ,LIU Wei ,GUO Hua.A Comparison between Methods for Detecting the Content of Carbohydrate with Honey of HPLC by Using Internal Standard Method and Chemiscal Method[J].Applied Science and Technology,2001,28(8):55-56,59. |
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| Authors: | WU Yan-Hua LIU Wei GUO Hua |
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| Institution: | WU Yan-Hua 1,LIU Wei 2,GUO Hua 3 |
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| Abstract: | There are a variety of carbohydrates in honey, but the time to detect is long and the detected contents of sucrose and glucose are possibly higher than the truth. With HPLC, fructose, glucose, sucrose and maltsugar can be directly separated and determined, the rhamnose is the internal standard and refractive index detector is the detector. This method is efficient, sensitive, accurate and convenient. |
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| Keywords: | high performance liquid chromatography internal standard fructose glucose sucrose maltsugar rhamnose honey |
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