| 短序落葵薯根多糖提取工艺及其生物活性 |
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| 引用本文： | 赵金莉,张亚如,顾晓阳,刘斌,金跃婷. 短序落葵薯根多糖提取工艺及其生物活性[J]. 河南师范大学学报(自然科学版), 2013, 41(2): 131-135 |
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| 作者姓名： | 赵金莉 张亚如 顾晓阳 刘斌 金跃婷 |
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| 作者单位： | 河北大学生命科学学院,河北保定,071002 |
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| 摘 要： | 通过正交试验研究短序落葵薯根多糖水提过程中提取温度、提取时间和料液比对多糖得率的影响,采用苯酚-硫酸法对多糖进行含量测定,选出较为理想的提取条件,即加20倍水,在100℃下浸提4h,得率15.72%.短序落葵薯根多糖能有效清除活性氧,对·OH,O2-,DPPH·的半清除率IC50分别为2.89,1.99和1.89mg/mL;对脂质过氧化的半抑制率为0.038mg/mL;在还原力的测定中,1mg/mL多糖在700nm下吸光值为0.696 7.
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| 关 键 词： | 短序落葵薯 根多糖 提取 生物活性 |
Extracting Technology Optimization of Polysaccharides from Anredera Scandens and Its Biological Activity |
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| ZHAO Jinli , ZHANG Yaru , GU Xiaoyang , LIU Bin , JIN Yueting. Extracting Technology Optimization of Polysaccharides from Anredera Scandens and Its Biological Activity[J]. Journal of Henan Normal University(Natural Science), 2013, 41(2): 131-135 |
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| Authors: | ZHAO Jinli ZHANG Yaru GU Xiaoyang LIU Bin JIN Yueting |
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| Affiliation: | (College of Life Sciences,Hebei University,Baoding 071002,China) |
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| Abstract: | The paper investgates the effect of extracting temperature,extracting time,and material-medium-rade on extraction of polysaccharides from stem of Anredera scandens with orthogonal design.The combination of adding 20 times of water,at the temperature of 100℃ and with etracting time of 4 h is recommended.Total polysaccharides in Anredera scandens was 15.72%.Root polysaccharides in Anredera scandens can effectively eliminate reactive oxygen species.The semi-clearance rate of ·OH-,O2-·and DPPH was 2.89 mg/mL,1.99 mg/mL and 1.89 mg/mL.The semi-inhibition rate of Lecith in lipid peroxidation was 0.038 mg/mL.Absorbance at 700 nm of reducing power was 0.6967 when the concentration of polysaccharide was 1mg/mL. |
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| Keywords: | Anredera scandens root polysaccharides extraction biological activity |
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