| 硫酸铵盐析在蕲蛇毒凝血酶样酶分离中的应用 |
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| 引用本文： | 郭春腾,宁千年,薛伟明,傅蓉,饶平凡.硫酸铵盐析在蕲蛇毒凝血酶样酶分离中的应用[J].福州大学学报(自然科学版),2002,30(1):122-125. |
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| 作者姓名： | 郭春腾 宁千年 薛伟明 傅蓉 饶平凡 |
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| 作者单位： | 1. 福州大学生物工程研究所,福建,福州,350002
2. 三明汇天生物药业有限公司,福建,三明,365000 |
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| 摘 要： | 探讨了硫酸铵沉降对蕲蛇毒中凝血酶样酶的初步分离效果的影响 ,确定出最佳处理条件 :2 .0 %的蕲蛇毒在pH 5 .0及 4 5 %饱和度的硫酸铵溶液中盐析沉降蕲蛇毒中非凝血酶样酶杂蛋白 ,上清液即为所分离的凝血酶样酶组分
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| 关 键 词： | 蕲蛇毒 凝血酶样酶 硫酸铵盐析 分离 |
| 文章编号： | 1000-2243(2002)01-0122-04 |
| 修稿时间： | 2001年7月4日 |
Application of ammonium sulfate salting-out to isolate thrombin-like enzymes from Agkistrodon acutus venom |
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| GUO Chun-teng,NING Qian-nian,XUE Wei-ming,FU Rong,RAO Ping-fan.Application of ammonium sulfate salting-out to isolate thrombin-like enzymes from Agkistrodon acutus venom[J].Journal of Fuzhou University(Natural Science Edition),2002,30(1):122-125. |
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| Authors: | GUO Chun-teng NING Qian-nian XUE Wei-ming FU Rong RAO Ping-fan |
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| Institution: | GUO Chun-teng 1,NING Qian-nian 2,XUE Wei-ming 1,FU Rong 1,RAO Ping-fan 1 |
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| Abstract: | The method of ammonium sulfate salting-out to preliminarily isolate thrombin-like enzymes from Agkistrodon acutus venom was applied and the separation effect was discussed in this paper. The optimum treatment to salting-out non-thrombin-like-enzyme components in 2.0% crude venom was determined as 45% ammonium sulfate at pH?5.0. The supernatant is preliminary isolated thrombin-like enzymes. The specific activity of the isolated thrombin-like enzymes is three times as high as rude Agkistrodon acutus venom, and percent recovery of protein is 54.6%. |
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| Keywords: | Agkistrodon acutus venom thrombin-like enzyme ammonium sulfate salting-out isolation |
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