| 淫羊藿甙对小鼠同种异基因皮片移植的影响 |
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| 引用本文： | 滕菲,曾耀英,黄秀艳,狄静芳,杨志.淫羊藿甙对小鼠同种异基因皮片移植的影响[J].暨南大学学报,2009,30(4). |
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| 作者姓名： | 滕菲 曾耀英 黄秀艳 狄静芳 杨志 |
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| 作者单位： | 暨南大学生命科学技术学院组织移植与免疫中心,广东,广州,510632 |
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| 基金项目： | "973"国家重点基础研究项目，广东省基金项目，广州市科技局科技攻关重点项目，生物化学与分子生物学广东省重点学科资助项目 |
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| 摘 要： | 目的:研究淫羊藿甙(ICA)对小鼠同种异基因皮片移植的影响.方法:试验分为腹腔注射生理盐水、ICA、环孢素A(CsA)以及CsA与ICA联用4组,5 d后将C57BL/C小鼠的背部皮片移植到BALB/c小鼠的相同位置,用眼科缝合线缝合,观察皮片愈合情况并记录皮片外观性状、排斥时间,于第11天取移植皮片做病理石蜡切片,取淋巴结细胞和脾细胞做增殖实验(CFDA-SE染色).结果:CsA+ICA联用能够延长皮片排斥时间,使皮片愈合得更好,胸腺和脾脏指数均比单独CsA组高,切片显示浸润的炎症细胞也较少,其淋巴结细胞增殖能力明显降低.结论:CsA与ICA联合能抑制移植造成的免疫排斥反应,可能是通过抑制受体小鼠的反应性T细胞的活化增殖来降低细胞免疫反应.
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| 关 键 词： | 淫羊藿甙 皮片移植 增殖 流式细胞术 |
Effect of icariin on allogeneic skin transplantation in mice |
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| TENG Fei,ZENG Yao-ying,HUANG Xiu-yan,DI Jing-fang,YANG Zhi.Effect of icariin on allogeneic skin transplantation in mice[J].Journal of Jinan University(Natural Science & Medicine Edition),2009,30(4). |
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| Authors: | TENG Fei ZENG Yao-ying HUANG Xiu-yan DI Jing-fang YANG Zhi |
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| Institution: | Institute of Tissue Transplantation and Immunology;College of Life Science and Techology;Jinan University;Guangzhou 510632;China |
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| Abstract: | Aim:To study the effect of icariin(ICA) on the rejection time of skin and proliferation of murine thymus and spleen cells. Methods: There are four groups in the experiment: peritoneal injection of saline group,ICA group,Cyclosporine A(CsA) group and CsA accompanied with ICA group.Five days later,the back skin grafts of C57BL/C mice were transplanted onto the same place of BALB/c mice and sewed up with ophthalmological sutural line.Observe the concrescence of skins and record their external characters and re... |
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