The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein

Summary A method to remove lipids from human plasma high-density lipoprotein (HDL) was developed. The procedure required column chromatography on Sephacryl-200 in the presence of the bile salt sodium deoxycholate. The lipid free protein obtained retained the immunological properties of the native HDL.The author is grateful to Ms L. Gray for her excellent technical assistance and Dr M. Fainaru of the Hadassah Medical Organization, Jerusalem, and Dr C. Heuck of the Klinisches Institut für Herzinfarktoforschung of Heidelberg, for kindly supplying purified apo-A, apo-A antiserum and apo-B antiserum.