微生物固定法降解含聚废水的最佳条件

利用从含聚废水中分离纯化得到的单一菌株R4-1,R4-2,R4-3,R4-5,H4-3,以海藻酸钠-PVA为包埋剂,采用包埋固定法对5种菌的混合菌进行固定化实验.通过单因素实验和正交实验,系统考察了湿菌体与包埋剂的体积比、氯化钙质量分数、硼酸质量分数和交联时间等因素对聚丙烯酰胺降解率的影响.实验得到微生物固定法降解含聚废水的最佳制备条件:4%海藻酸钠,4%的PVA,湿菌体与包埋剂体积比1∶1,2%氯化钙,3%硼酸,分段交联时间为氯化钙4h后硼酸20h.湿菌体与包埋剂的体积比对聚丙烯酰胺降解率的影响十分显著.追加实验最佳制备条件,得到微生物固定化颗粒对含聚废水的降解率可达到83.1%.

Optimization of Degradation Conditions of Poly-containing Wastewater by Immobilized Microorganism

Strains R4-1,R4-2,R4-3,R4-5,H4-3 were obtained by separation and purification experiment from poly-containing wastewater,Sodium Alginate-PVA as embedding medium,immobilized experiment research of mixed bacteria group were studied by Embedding fixed method.The volume ratio of wet bacterium to embedding medium,the mass fraction of calcium chloride,the mass fraction of boric acid and the immobilized time on degrading poly-containing wastewater were investigated by experiment of single factor and orthogonal experiment.The optimum process conditions of degrading poly-containing wastewater by immobilize microorganism are as follows: the mass fraction of sodium alginate is 4%;the mass fraction of PVA is 4%;the volume ratio of wet bacterium to embedding medium is 1∶1;the mass fraction of calcium chloride is 2%;the mass fraction of boric acid is 3%;the immobilize time is 4 h immobilization in calcium chloride solution and then 20 h immobilization in boric acid solution.The main factor affecting microbial immobilization was volume ratio of wet bacterium to embedding medium.Experiment subjoined by optimum process.Degradation rate of immobilization grains degrading poly-containing wastewater is 83.1%.