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生物素对红发夫酵母生长和虾青素积累的影响
引用本文:朱明军,梁世中. 生物素对红发夫酵母生长和虾青素积累的影响[J]. 华南理工大学学报(自然科学版), 2003, 31(6): 80-84
作者姓名:朱明军  梁世中
作者单位:华南理工大学,食品与生物工程学院,广东,广州,510640
基金项目:广东省自然科学基金(990671),华南现工大学自然科学基金(E5321277)
摘    要:在摇瓶中研究生物素对红发夫酵母生长和虾青素积累的影响.结果表明生物素的添加能促进细胞生长,但对细胞内虾青素含量的提高不利.添加10μg/L生物素时,最大比生长速率达到0.110h-1;添加6μg/L生物素时,达到最大细胞干重9.95g/L,比没有添加生物素时的7.53g/L提高32%.添加2μg/L生物素时,达到最高虾青素产量和产率,分别为3825 μg/L和53μg/(L@h),比没有添加生物素时的2 944 μg/L和41μg/(L@h)提高30%.没有添加生物素时,达到最高细胞虾青素含量391μg/g.利用添加2μg/L生物素的方法可以提高虾青素产量,这种方法为提高虾青素产量提供了一种简单廉价的方法.

关 键 词:生物素 虾青素 红发夫酵母

Effects of Biotin on Cell Growth and Astaxanthin Accumulation of Phaffia Rhodozyma
Abstract. Effects of Biotin on Cell Growth and Astaxanthin Accumulation of Phaffia Rhodozyma[J]. Journal of South China University of Technology(Natural Science Edition), 2003, 31(6): 80-84
Authors:Abstract
Abstract:The effects of biotin on cell growth and astaxanthin accumulation of Phaffia rhodozyma were investigated in flasks. The addition of biotin promoted cell growth but decreased the cell astaxanthin content.The highest specific growth rate of 0. 110 h-1 was achieved at 10 μg/L biotin. The highest cell dry weight of 9.95 g/L was achieved at 6 μg/L biotin, which increased by 32% compared with 7.53 g/L when there was no biotin. As for astaxanthin production, the highest yield of astaxanthin of 3 825 μg/L and productivity of 53 μg/(L @ h) were reached at 2 μg/L biotin, which increased by 30% compared with 2 944 μg/L and 41 μg/(L @ h) with the absence of biotin, while the highest astaxanthin content of 391 μg/g was reached at 0 μg/L biotin. This method provides a simple and inexpensive means to increase astaxanthin production in industrial fermentations.
Keywords:biotin  astaxanthin  Phaffia rhodozyma
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