| 微乳液电动毛细管色谱-激光诱导荧光法测定中药和风湿性心脏病患者血浆中的肾上腺素和多巴胺 |
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| 引用本文： | 董钰明,陈晓峰,李春兰,陈兴国. 微乳液电动毛细管色谱-激光诱导荧光法测定中药和风湿性心脏病患者血浆中的肾上腺素和多巴胺[J]. 兰州大学学报(自然科学版), 2009, 45(3) |
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| 作者姓名： | 董钰明 陈晓峰 李春兰 陈兴国 |
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| 作者单位： | 兰州大学药学院,兰州,730000;兰州大学化学化工学院,兰州,730000;兰州大学化学化工学院,兰州,730000;兰州大学学报编辑部,兰州,730000;甘肃省干部保健医院心内科,兰州,730020;兰州大学化学化工学院,兰州,730000 |
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| 基金项目： | 国家自然科学基金，Natural Science Foundation of GansuProvince |
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| 摘 要： | 建立了微乳液毛细管电动色谱-激光诱导荧光法测定用NBD-Cl衍生后的肾上腺素和多巴胺的方法.微乳液母液的组成为w(SDS):w(1-丁醇):2(n-庚烷):w(水)=3.31:6.61:0.80:89.28.优化后的分离条件为:20 mmol/L硼酸钠+体积分数为20%的微乳液+体积分数为4%的乙腈(调pH至9.3)组成缓冲液,分离电压20 kV,峰面积和分析物浓度之间有良好的线性关系.应用本方法测定2种中药和风湿性心脏病患者血浆中的肾上腺索和多巴胺,回收率为95.8%～105.6%.
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| 关 键 词： | 微乳液电动毛细管色谱 激光诱导荧光 肾上腺素 多巴胺 |
MEEKC with laser induced fluorescence detection of epinephrine and dopamine in TCM and in plasma of patients with rheumatic heart disease |
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| DONG Yu-ming,CHEN Xiao-feng,LI Chun-lan,CHEN Xing-guo. MEEKC with laser induced fluorescence detection of epinephrine and dopamine in TCM and in plasma of patients with rheumatic heart disease[J]. Journal of Lanzhou University(Natural Science), 2009, 45(3) |
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| Authors: | DONG Yu-ming CHEN Xiao-feng LI Chun-lan CHEN Xing-guo |
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| Affiliation: | 1;2;2;3;1.School of Pharmacy;Lanzhou University;Lanzhou 730000;China;2.College of Chemistry and Chemical Engineering;3.Editorial Board of Journal of Lanzhou University;4.Department of Internal Cardiology;Cadre Medical Care Hospital of Gansu Province;Lanzhou 730020;China |
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| Abstract: | A microemulsion electrokinetic chromatography method with laser induced fluorescence detection was established to analyze epinephrine and dopamine after their having been derivatizated with 4-chloro -7-nitrobenzo-2-oxa-1,3-diazol.A stock microemulsion solution composed of w(SDS):w(1-butanol): w(n-heptane):w(water)=3.31:6.61:0.80:89.28 was prepared.The optimum separation conditions were as follows:the running buffer was composed of 20 mmol/L borate+20%microemulison(V/V)+4%acetonitrile (adjust pH to 9.3) and ... |
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| Keywords: | microemulsion electrokinetic chromatography laser induced fluorescence detection epineph-rine dopamine |
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