| TBSV病毒瞬时表达载体构建及其表达 |
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| 引用本文： | 王盛,王杨,李志英,张虹,雒丽娜,董洁,王玉炯.TBSV病毒瞬时表达载体构建及其表达[J].宁夏大学学报(自然科学版),2011,32(2):159-163. |
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| 作者姓名： | 王盛 王杨 李志英 张虹 雒丽娜 董洁 王玉炯 |
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| 作者单位： | 1. 宁夏大学生命科学学院,宁夏银川750021;宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021
2. 宁夏大学生命科学学院,宁夏银川,750021 |
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| 基金项目： | 国家自然科学基金资助项目(3016002); 教育部科学技术研究重点基金资助项目(209135); 宁夏高等学校科学技术研究基金资助项目 |
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| 摘 要： | 为了深入理解植物RNA病毒载体表达系统,初步建立了利用TBSV病毒表达载体在寄主植物烟草中表达外源蛋白的技术体系.以番茄丛矮病毒(Tomato bushy stunt virus,TBSV)为材料,构建了含报告基因sGFP的WY1,WY2等2个重组病毒表达载体,并将载体导入农杆菌GV3101用于侵染烟草植株叶片;利用农...
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| 关 键 词： | 植物病毒 番茄丛矮病毒 表达载体 瞬时表达 烟草 |
Construction of TBSV RNA-Based Transient Expression Vectors and Its Expression in Tobacco Plants |
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| Wang Sheng,Wang Yang,Li Zhiying,Zhang Hong,Luo Lina,Dong Jie,Wang Yujiong.Construction of TBSV RNA-Based Transient Expression Vectors and Its Expression in Tobacco Plants[J].Journal of Ningxia University(Natural Science Edition),2011,32(2):159-163. |
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| Authors: | Wang Sheng Wang Yang Li Zhiying Zhang Hong Luo Lina Dong Jie Wang Yujiong |
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| Institution: | Wang Sheng1,2,Wang Yang1,Li Zhiying1,Zhang Hong1,Luo Lina1,Dong Jie1,Wang Yujiong1,2(1.School of Life Science,Ningxia University,Yinchuan 750021,China,2.Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China,China) |
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| Abstract: | In order to better understand the utility of plant virus RNA-based expression system,we established a TBSV RNA-based expression system for transient expression of proteins in tobacco.Constructs were generated with full-length Tomato bushy stunt virus(TBSV) cDNA and two TBSV-derived expression vectors(WY1 and WY2) were constructed.Those vectors were delivered into Tobacco plants(Nicotiana benthamiana)by means of an agrobacterium-mediated transient transformation assay.The results show that the fluorescent si... |
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| Keywords: | tomato bushy stunt virus TBSV expression vector transient expression tobacco |
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