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TBSV病毒瞬时表达载体构建及其表达
引用本文:王盛,王杨,李志英,张虹,雒丽娜,董洁,王玉炯.TBSV病毒瞬时表达载体构建及其表达[J].宁夏大学学报(自然科学版),2011,32(2):159-163.
作者姓名:王盛  王杨  李志英  张虹  雒丽娜  董洁  王玉炯
作者单位:1. 宁夏大学生命科学学院,宁夏银川750021;宁夏大学西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021
2. 宁夏大学生命科学学院,宁夏银川,750021
基金项目:国家自然科学基金资助项目(3016002); 教育部科学技术研究重点基金资助项目(209135); 宁夏高等学校科学技术研究基金资助项目
摘    要:为了深入理解植物RNA病毒载体表达系统,初步建立了利用TBSV病毒表达载体在寄主植物烟草中表达外源蛋白的技术体系.以番茄丛矮病毒(Tomato bushy stunt virus,TBSV)为材料,构建了含报告基因sGFP的WY1,WY2等2个重组病毒表达载体,并将载体导入农杆菌GV3101用于侵染烟草植株叶片;利用农...

关 键 词:植物病毒  番茄丛矮病毒  表达载体  瞬时表达  烟草

Construction of TBSV RNA-Based Transient Expression Vectors and Its Expression in Tobacco Plants
Wang Sheng,Wang Yang,Li Zhiying,Zhang Hong,Luo Lina,Dong Jie,Wang Yujiong.Construction of TBSV RNA-Based Transient Expression Vectors and Its Expression in Tobacco Plants[J].Journal of Ningxia University(Natural Science Edition),2011,32(2):159-163.
Authors:Wang Sheng  Wang Yang  Li Zhiying  Zhang Hong  Luo Lina  Dong Jie  Wang Yujiong
Institution:Wang Sheng1,2,Wang Yang1,Li Zhiying1,Zhang Hong1,Luo Lina1,Dong Jie1,Wang Yujiong1,2(1.School of Life Science,Ningxia University,Yinchuan 750021,China,2.Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China,China)
Abstract:In order to better understand the utility of plant virus RNA-based expression system,we established a TBSV RNA-based expression system for transient expression of proteins in tobacco.Constructs were generated with full-length Tomato bushy stunt virus(TBSV) cDNA and two TBSV-derived expression vectors(WY1 and WY2) were constructed.Those vectors were delivered into Tobacco plants(Nicotiana benthamiana)by means of an agrobacterium-mediated transient transformation assay.The results show that the fluorescent si...
Keywords:tomato bushy stunt virus  TBSV  expression vector  transient expression  tobacco  
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