| Neural Stem Cell Affinity of Chitosan and Feasibility of ChitosanBased Porous Conduits as Scaffolds for Nerve Tissue Engineering |
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| 作者姓名： | 王爱军 敖强 贺庆 巩晓明 龚锴 公衍道 赵南明 张秀芳 |
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| 作者单位： | State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，Yuquan Hospital Tsinghua University Beijing 100049 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，Medical School Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China，Medical School Tsinghua University Beijing 100084 China，State Key Laboratory of Biomembrane and Membrane Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (No. 30400099), the National Key Basic Research and Develop-ment (973) Program of China (No. 2005CB623905), and the Tsinghua-Yue-Yuen Medical Science Fund |
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| 摘 要： | Introduction Neural stem cells (NSCs) are known to have both self- proliferation potential and multiple differentiation po- tential1]. Previous studies have shown that NSCs can differentiate into various cell types such as neurons, as- trocytes, oligoden…
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| 关 键 词： | 神经干细胞 壳聚糖 神经管 组织工程 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-09-212005-11-30 |
Neural Stem Cell Affinity of Chitosan and Feasibility of Chitosan-Based Porous Conduits as Scaffolds for Nerve Tissue Engineering |
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| WANG Aijun,AO Qiang,HE Qing,GONG Xiaoming,GONG Kai,GONG Yandao,ZHAO Nanming,ZHANG Xiufang.Neural Stem Cell Affinity of Chitosan and Feasibility of ChitosanBased Porous Conduits as Scaffolds for Nerve Tissue Engineering[J].Tsinghua Science and Technology,2006,11(4):415-420. |
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| Authors: | WANG Aijun AO Qiang HE Qing GONG Xiaoming GONG Kai GONG Yandao ZHAO Nanming ZHANG Xiufang |
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| Institution: | WANG Aijun,AO Qiang,HE Qing,GONG Xiaoming,GONG Kai,GONG Yandao,ZHAO Nanming,ZHANG Xiufang(1. State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China; 2. Yuquan Hospital, Tsinghua University, Beijing 100049, China; 3. Medical School, Tsinghua University, Beijing 100084, China |
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| Abstract: | Neural stem cells (NSCs) are currently considered as powerful candidate seeding cells for regen- eration of both spinal cords and peripheral nerves. In this study, NSCs derived from fetal rat cortices were co-cultured with chitosan to evaluate the cell affinity of this material. The results showed that NSCs grew and proliferated well on chitosan films and most of them differentiated into neuron-like cells after 4 days of culture. Then, molded and braided chitosan conduits were fabricated and characterized for their cytotoxicity, swelling, and mechanical properties. Both types of conduits had no cytotoxic effects on fibroblasts (L929 cells) or neuroblastoma (Neuro-2a) cells. The molded conduits are much softer and more flexible while the braided conduits possess much better mechanical properties, which suggests different potential applications. |
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| Keywords: | neural stem cells chitosan nerve conduit tissue engineering |
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