坛紫菜种质资源遗传多样性的AFLP分析

利用AFLP技术从基因组DNA水平上分析福建省主要坛紫菜栽培品系和诱变筛选获得的突变品系的遗传差异,在25对引物中,有11对能得到重复性好的多态性扩增条带,总共在667个位点能扩增出条带,每对引物扩增带数为24~90条,并且各对引物扩增结果均显示样品间存在差异.共得到522条多态性条带,占总扩增带的78.3%.根据AFLP图谱计算了不同样品间的遗传距离(D)和相似性系数(GS).优良品系GL和CCS之间的遗传距离最小,仅为0.264,不同样品间的相似性系数介于0.602~0.736之间.并根据D值绘制了它们的UPGMA聚类图谱.

Analysis of Genetic Diversity of Porphyra haitanensis Using AFLP

Amplified fragment length polymorphic technique was used to estimate the genomic DNA polymorphism among the main strains of Porphyra haitanensis in Fujian Province and four mutation strains.11 of the 25 AFLP primer pairs gave reproducible polymorphic DNA amplification patterns,and were selected to construct a DNA fingerprinting table to estimate the genetic diversity among these samples.The amplified bands ranged from 24 to 90 per primer pair.667 AFLP markers were obtained and 522 of them were polymorphic loci.The proportion of polymorphic loci was 78.3%.The genetic distance(D) and similarity coefficients(GS) were calculated based on the polymorphic data.The GS between these samples was 0.602~0.736,while the D between GL and CCS was only 0.264.A dendrogram by unweighted pair group method with arithmetic means was constructed according to the D value.