| CTAB法提取动物DNA |
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| 引用本文： | 赵瑞杰,严海燕,谭艳平,王朝元,林爱华. CTAB法提取动物DNA[J]. 中国西部科技, 2011, 10(21): 7-8. DOI: 10.3969/j.issn.1671-6396.2011.21.004 |
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| 作者姓名： | 赵瑞杰 严海燕 谭艳平 王朝元 林爱华 |
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| 作者单位： | 中南民族大学生命科学院,湖北武汉,430073 |
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| 摘 要： | 针对实际操作中常用的动物基因组DNA提取方法所面临的一些不足,对CTAB法进行改进,将其运用于动物基因组DNA的提取。结果表明,改进后的CTAB法具有时间短、质量高、成本低的优点,与实验教学中植物DNA和RNA提取的方法相一致,使分子生物学教学用品一体化,价格低廉化,效果改进,是一个不仅可以用于研究,也可以大规模用于实验教学的好方法。
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| 关 键 词： | DNA提取 CTAB法 动物基因组 |
Animal DNA Extraction with CTAB Method |
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| ZHAO Rui-jie,YAN Hai-yan,TAN Yan-ping,WANG Cao-yuan,LIN Ai-hua. Animal DNA Extraction with CTAB Method[J]. Science and Technology of West China, 2011, 10(21): 7-8. DOI: 10.3969/j.issn.1671-6396.2011.21.004 |
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| Authors: | ZHAO Rui-jie YAN Hai-yan TAN Yan-ping WANG Cao-yuan LIN Ai-hua |
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| Affiliation: | ZHAO Rui-jie,YAN Hai-yan,TAN Yan-ping,WANG Cao-yuan,LIN Ai-hua (College of life Sciences,South-central Universities for Nationalities,Wuhan,Hubei 430073) |
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| Abstract: | Due to some defects existed in DNA extraction method from animal tissues used in routine lab work,an improved CTAB was used in genomic DNA extraction from animal tissues.The results indicated that the improved CTAB method had privileges of short time span,higher quality of DNA product,low cost,and convenient for molecular biology lab teaching,in which the same method was also used in DNA and RNA extraction from plant,making it a systematic method in the lab teaching.And thus,CTAB method was a method not onl... |
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| Keywords: | DNA extraction CTAB method Animal genomic DNA |
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