厚朴组织培养与高产细胞系建立的研究

对来自厚朴种源试验林的10个不同基因型材料开展组织培养和高产细胞系,结果表明：基本培养基、激素种类与配比、外植体的取材部位等因素对厚朴愈伤组织的诱导率、生化、褐化均有显影响。B5＋2，4-D 4mg/L NAA 1mg/L培养基的诱导率达到100％；而采用B5＋BA 1.0-2.0mg/L NAA 1.0MG/l的培养基，愈伤组织的增殖率最高，褐化率最低；140、54两个基因型的细胞增殖最快，是其它基因型细胞均值的2．12倍；不同的基因型、培养基、培养时间所产生愈伤组织细胞的厚朴酚类含量不同，以140基因型的细胞系在B5＋BA 2.0mg/L NAA 1.0mg/L的培养基上培养60d为最优。

Studies on Tissue Culture and the Establishment of a High-Yield Cell Line of Magnolia officinalis

In this paper,techniques for induction of callus and cell culture in Magnolia officinalis have been studied,with an aim to establish a cell line in which cells proliferated fast and phenol content was high.Based on tissue culture and the establishment of a high yield cell line of 10 genotypes from a provenance test forest of Magnolia officinalis ,it shows that such factors as basic media,types and proportions of hormones,and explants had a great effect on the induction rate,growth and browning of the callus.With a medium of B 5 +4 mg/L 2,4 D+1 mg/L NAA,the induction rate reached 100%.With the medium of B 5 +1.2-2.0 mg/L BA+1.0 mg/L NAA,the callus had a highest proliferation rate and a lowest percentage of browning.Cells of the two genotypes, viz.140 and 54 grew fastest and were 2.12 times as many as those of other genotypes on the average.The content of phenols differed between calluses cultured from different genotypes and media as well as for a different period of time.The best one was the cell line of the genotype 140 cultured on the medium of B 5 +2.0 mg/L BA+ 1.0 mg/L NAA for 60 days.