基于鲁米诺功能化纳米金的电化学发光适配体传感器灵敏检测癌胚抗原

以鲁米诺功能化的纳米金(L-Au NPs)为发光剂,H_2O_2为共反应剂所构成的电化学发光(ECL)体系具有优良的ECL性能.利用L-Au NPs为电极基底材料,具有特异性识别作用的适配体为识别元素,构建ECL适配体传感器,发展了一种灵敏检测癌胚抗原(CEA)的新方法.结果表明,CEA的线性范围为5.0×10~(-10)~5.0×10~(-6)g/L,检出限为1.8×10~(-10)g/L(R_(SN)=3).将提出的方法应用于人血清样品中CEA含量的检测,检测结果与酶联免疫分析方法吻合,相对误差不大于2.9%.加标回收率在85.3%~113.6%之间.该研究为疾病标志物灵敏检测提供了一种新方法.

Electrochemiluminescence Aptasensor for Sensitive Detection of Carcinoembryonic Antigen Based on Luminol-Functionalized Gold Nanoparticles

The luminol-functionalized gold nanoparticles( L-Au NPs) exhibited excellent electrochemiluminesent( ECL) performance. In this work,a new ECL aptasensor for sensitive detection of cacinoembryonic antigen( CEA)was constructed with the L-Au NPs as base material and the aptamer with specific recognition as the recognizing element. The ECL aptasensor for CEA detection exhibitsed a linear range from 5.0×10~(-10) to 5.0×10~(-6) g/L and the detection limit was 1.8×10~(-10) g/L( R_(SN)= 3). Moreover,the proposed method was applied to determine the CEA in human serum samples with the recovery of 85.3% ~ 113.6%,and the relative error was less than 2.9% compared with the enzyme-linked immunoassay method. The studies supplied a general methodology to enhance ECL efficiency and showed a promising application prospects in the field of biological detection.