Discovery of a male-biased mutant family and identification of a male-specific SCAR marker in gynogenetic gibel carp Carassius auratus gibelio |
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Authors: | Da Wang Huiling Mao Jinxia Peng Xiyin Li Li Zhou Jianfang Gui |
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Affiliation: | 1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences,Graduate School of Chinese Academy of Sciences, VVuhan 430072, China 2. School of Life Science, Nanchang University, Nanchang 330031, China |
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Abstract: | Gibel carp (Carassius auratus gibelio) is a uniquely gynogenetic species with a minor ratio of males in natural habitats, but its male origin and sex determination mechanisms have been unknown. In this study, a male-biased mutant family was discovered from the gynogenetic gibel carp, and a male-specific SCAR marker was identified from the mutant family. Normal spermatogenesis was observed in the male testes by immunofluorescence histochemistry. Nearly identical AFLP profiles were observed between males and females, but a male-specific 86 bp AFLP fragment was screened by sex-pool bulked segregant analysis and individual screening. Based on the male-specific AFLP fragment, a total of 579 bp sequences were cloned by genome walking. Subsequently, a male-specific SCAR marker was designed, and the male-specific DNA fragment was confirmed to be steadily transmitted to the next generation and consistently detected only in males. |
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Keywords: | Gibel carp (Carassius auratus gibelio) Crynogenesis Male-biased mutant family Male-speeitic SCAR marker (MSM) |
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