Molecular characterization of RAPD and SCAR marker linked to the frog-eye leaf spot resistance gene in soybean |
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| Authors: | Jijun Zou Qingkai Yang Shouyi Chen Qingshan Chen Yaguang Liu Wei Dong |
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| Institution: | (1) Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Soybean Research Institute, Northeast Agricultural University, 150030 Harbin, China |
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| Abstract: | Two fragments SCS3620 and SCS3580 of the co-dominant marker OPS03620 & 580 that were linked to the resistance gene of soybean frog-eye leaf spot have been completely sequenced. A significant insertion
of 30 bp is the main reason of the polymorphism between the two fragments. The results of Southern hybridization indicate
that SCS3620 derives from a single-or low-copy sequence and can be used as an RFLP probe. A co-dominant SCAR marker SCS3620 & 580 has been developed based on the sequences. The segregation of SCS3620 & 580 is similar to that of RAPD marker OPS03620 & 580-Significant polymorphism has been shown between resistant and susceptible genotypes when 62 soybean genotypes were surveyed
for the SCAR marker. Therefore, the marker can be used in the resistance breeding of soybean frog-eye leaf spot by marker-assisted
selection. |
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| Keywords: | Cercospora sojina Hara disease resistance molecular markers co-dominance |
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