| 水稻染色体微切割及4号染色体酶解片段的扩增 |
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| 引用本文： | 邓可京,何风华.水稻染色体微切割及4号染色体酶解片段的扩增[J].复旦学报(自然科学版),1999,38(4):444-446,450. |
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| 作者姓名： | 邓可京 何风华 |
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| 作者单位： | 复旦大学遗传学研究所 |
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| 基金项目： | 国家自然科学基金，复旦大学青年自然科学基金 |
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| 摘 要： | 通过细胞分裂的同步化，低渗，酶消化，瞬间固定和涂片等制片技术，快速制备水稻根尖体细胞染色体标本。该方法制备出的染色体标本，杂质少，染色体分散程度好，满足了染色体微切割用片的要求。染色体微切割和ＰＣＲ扩增技术，获得了水稻第４号染色体ＤＮＡ文库。
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| 关 键 词： | 水稻 染色体 制片 微切割 PCR扩增 |
Microdissection and Amplification of Rice Chromosome No.4 |
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| Deng Kejing,He Fenghua,Shen Daleng.Microdissection and Amplification of Rice Chromosome No.4[J].Journal of Fudan University(Natural Science),1999,38(4):444-446,450. |
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| Authors: | Deng Kejing He Fenghua Shen Daleng |
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| Institution: | Institute of Genetics |
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| Abstract: | By the treatments of synchronization of meristematic cell division in root tips,hypotonic method,transient fixation, enzyme digestion and smearing technique, well spreaded and low contaminated rice chromosomes were quickly prepared and fitted for chromosome microdissection. No.4 chromosomal DNA library was constructed by means of chromosome microdissection and PCR technique. It was primarily confirmed by FISH technique that the DNA library was really from rice cell nuclei. It will be helpful for cloning plant genes located in specific chromosome or related region. |
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| Keywords: | rice chromosome quick preparation of chromosome slide microdissection primer linker PCR amplification |
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