| 嗜热菌Lon蛋白酶的功能分析 |
| |
| 引用本文： | 王昕,刘晓晴.嗜热菌Lon蛋白酶的功能分析[J].首都师范大学学报(自然科学版),2009,30(5):35-39,53. |
| |
| 作者姓名： | 王昕 刘晓晴 |
| |
| 作者单位： | 首都师范大学生命科学学院,北京,100048 |
| |
| 基金项目： | 国家自然科学基金资助 |
| |
| 摘 要： | Lon蛋白酶是一种在各种生物体内广泛分布并且具有多种生物功能的蛋白质.以嗜热栖热菌（Thermus thermophilus HB8）的Lon蛋白酶（TTLon）为研究对象,将TTLon蛋白酶的编码基因克隆至pTrc His载体,并在大肠杆菌中进行了成功表达,采用Ni-NTA亲和层析对其进行分离纯化,并通过一系列实验证明Lon蛋白酶能够与ATP结合,具有依赖ATP的蛋白酶活性和依赖ATP的分子伴侣活性.
|
| 关 键 词： | Lon蛋白酶 ATP依赖型蛋白酶 分子伴侣. |
Characterization of Lon Protease from Thermus thermophilus HB8 |
| |
| Wang Xin,Liu Xiaoqing.Characterization of Lon Protease from Thermus thermophilus HB8[J].Journal of Capital Normal University(Natural Science Edition),2009,30(5):35-39,53. |
| |
| Authors: | Wang Xin Liu Xiaoqing |
| |
| Institution: | College of Life Science;Capital Normal University;Beijing 100048 |
| |
| Abstract: | Lon proteases are conserved in all living organisms and play important biological roles.With the material of Lon protease from Thermus thermophilus HB8,the gene was cloned and connected to the plasmid pTrcHis.TTLon protease was successfully expressed in E.coli.The recombinant protein was purified with affinity chromatography(Ni-NTA).A series of experiments showed that the TTLon can bind with ATP,the activity of protease and the activity of molecular chaperone were both ATP-dependent. |
| |
| Keywords: | Lon protease ATP-dependent protease molecular chaperone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
