高效液相色谱-四级杆飞行时间串联质谱分析3种荷花的花色苷

应用高效液相色谱-电喷雾/四极杆飞行时间串联质谱联用技术（HPLC-ESI/Q-TOF-MS/MS）分析了3种荷花（大红袍、洪湖红莲、红牡丹）的花色苷成分。首先以φ_HCl= 0.1%的甲醇超声提取荷花中的花色苷，然后用XAD-7大孔树脂纯化，选用Amethyst C18色谱柱(250 mm×4.6 mm，5 μm)，以甲醇-水（φ_C2HF3O2=0.05%）为流动相进行梯度洗脱，柱后流出液采用电喷雾四极杆飞行时间质谱(ESI/Q TOF MS/MS)进行检测，共分离鉴定出6种花色苷，并且运用pH示差法分别测定了3种荷花中总花色苷的含量，为开发利用这一丰富的自然资源提供一定的参考依据。

Analysis of anthocyanins in three species Nelumbinis flos. by HPLC- Electrospray Ionization/Quadrupole-Time of Flight-Mass/Mass Spectrometry

We employed HPLC-Electrospray Ionization/Quadrupole-Time of Flight-Mass/Mass Spectrometry(HPLC-ESI/Q-TOF-MS/MS) to analyze the anthocyanins in three species Nelumbo nucifera.The anthocyanins were extracted with methanol containing HCl(φHCl= 0.1%) and then purified on an Amberlite XAD-7 column.We applied an Amethyst C18 column(250 mm×4.6 mm,5 μm) to HPLC analysis with water methanol-water(containing trifluoroacetic acid(φC2HF3O2=0.05%)) in gradient as mobile phase.The eluates were then detected by ESI/Q-TOF-MS/MS.We altogether identified six species anthocyanins from these Nelumbo nucifera.We further determined their contents by pH-differential spectrophotometry.It provides theoretical foundation for further development and utilization of Nelumbo nucifera resources.