| 应用RAPD和同工酶遗传标记鉴别彭泽鲫中的克隆 |
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| 引用本文： | 董仕,王茜,乔之怡. 应用RAPD和同工酶遗传标记鉴别彭泽鲫中的克隆[J]. 集美大学学报(自然科学版), 2003, 8(3): 208-212 |
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| 作者姓名： | 董仕 王茜 乔之怡 |
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| 作者单位： | 1. 天津师范大学化学与生命科学学院,天津,300074
2. 天津农学院水产科学系,天津,300384 |
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| 基金项目： | 教育部留学人员科研启动基金 |
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| 摘 要： | 应用随机扩增多态性DNA(RAPD)和同工酶检测技术检测分析了18尾彭泽鲫，从样品鳍条提取基因组DNA，水平式淀粉凝胶电泳法检测同工酶，结果显示，肝脏GPI、肌肉GPI和肝脏EST3种同工酶均有变异出现，3种同工酶组合后可将实验鱼划分为遗传组成各不相同的8种克隆，6种引物PCR扩增的RAPD电泳图谱与同工酶的划分结果一致。
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| 关 键 词： | 彭泽鲫 基因克隆 随机扩增多态性DNA RAPD 同工酶 遗传标记 遗传组成 |
| 文章编号： | 1007-7405(2003)03-0208-05 |
| 修稿时间： | 2003-05-07 |
Identification of Clone in Pengze Crucian Carp, C. auratus pengzesis Using Isozyme and RAPD Markers |
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| DONG Shi,WANG Qian,QIAO Zhi-yi. Identification of Clone in Pengze Crucian Carp, C. auratus pengzesis Using Isozyme and RAPD Markers[J]. the Editorial Board of Jimei University(Natural Science), 2003, 8(3): 208-212 |
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| Authors: | DONG Shi WANG Qian QIAO Zhi-yi |
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| Abstract: | Genetic structure of pengze crucian carp was examined by using isozyme and RAPD genetic markers. The DNA samples were extracted from the caudal fin ray of samples. The isozymes were examined by using starch gel. 18 samples were divided into 8 clonal types by patterns of GPI (liver) , GPI (muscle) and EST (liver) isozymes. The results of RAPD were the same with the results of isozymes. |
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| Keywords: | pengze crucian carp clone isozyme RAPD |
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