| 多胺化壳聚糖载体固定化漆酶及其性质研究 |
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| 引用本文： | 周雪,朱启忠. 多胺化壳聚糖载体固定化漆酶及其性质研究[J]. 河南师范大学学报(自然科学版), 2011, 39(5): 125-128 |
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| 作者姓名： | 周雪 朱启忠 |
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| 作者单位： | 山东大学威海分校海洋学院,山东威海,264209 |
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| 摘 要： | 以多胺化壳聚糖为载体,戊二醛为交联剂固定化白毒鹅膏菌漆酶,研究了最佳固定化条件及固定化酶的部分性质.结果表明,戊二醛浓度2％,交联时间14h,固定化时间6h,给酶最70mg/g载体时同定化效果最佳,酶活力可达64.3 U/g.此固定化漆酶的Km为8.73mmol· L-1；最适pH值4.2,较自由漆酶向酸性偏移；最适反...
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| 关 键 词： | 多胺化壳聚糖 同定化 漆酶 |
Immobilization and Characteristics of Fungal Laccase on Polyethyleneaminated Chitosan |
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| ZHOU Xue,ZHU Qi-zhong. Immobilization and Characteristics of Fungal Laccase on Polyethyleneaminated Chitosan[J]. Journal of Henan Normal University(Natural Science), 2011, 39(5): 125-128 |
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| Authors: | ZHOU Xue ZHU Qi-zhong |
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| Affiliation: | ZHOU Xue,ZHU Qi-zhong(Marine College of Shangdong University at Weihai,Weihai 264209,China) |
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| Abstract: | Laccase has been immobilized on polyethyleneaminated chitosan with glutaraldehyde as crosslinking agent.When glutaraldehyde concentration is 2%,linking time is 14 h,immobilization time is 6 h and laccase dosage is 70 mg/g carrier the optimal condition was obtained.In this way 64 U/g of immobilized enzyme was available.The Km for the immobilized enzyme is 8.73 mmol·L-1 and the optimal pH and temperature for immobilized enzyme are 4.2 and 50 ℃.Compared with the free enzyme,its stability is considerable improved. |
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| Keywords: | polyethyleneaminated chitosan immobilization fungal laccase |
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