| 果蝇nulp1特异性多克隆抗体制备及检测 |
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| 引用本文： | 龚琳,王跃群,袁婺洲,莫小阳,万永奇,江志钢,吴秀山,李永青.果蝇nulp1特异性多克隆抗体制备及检测[J].湖南师范大学自然科学学报,2011,34(4):69-73. |
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| 作者姓名： | 龚琳 王跃群 袁婺洲 莫小阳 万永奇 江志钢 吴秀山 李永青 |
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| 作者单位： | 湖南师范大学蛋白质化学与发育生物学教育部重点实验室,心脏发育研究中心,中国长沙410081 |
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| 基金项目： | 国家自然科学基金资助项目(30930054,30871417,30971663); 国家重点基础研究发展计划资助项目(2005CB522505); 湖南省自然科学基金资助项目(09JJ5014) |
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| 摘 要： | 为了利用果蝇模型研究nulp1蛋白在早期心脏发育中的功能,采用PCR技术扩增出果蝇nulp1基因的部分编码区并插入到pET28a表达载体中.之后将重组质粒转入大肠杆菌(E.coli)Rosetta( DE3)；通过IPTG诱导表达His-nulpl融合蛋白,该融合蛋白采用Ni2+金属螯合层析纯化后,免疫新西兰兔制备了多...
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| 关 键 词： | nulp1 果蝇 原核表达 多克隆抗体 |
Polyclonal Antibody Preparation of Nulp1 Gene in Drosophila |
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| GONG Lin,WANG Yue-qun,YUAN Wu-zhou,MO Xiao-yang,WAN Yong-qi,JANG Zhi-gang,WU Xiu-shan,LI Yong-qing.Polyclonal Antibody Preparation of Nulp1 Gene in Drosophila[J].Journal of Natural Science of Hunan Normal University,2011,34(4):69-73. |
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| Authors: | GONG Lin WANG Yue-qun YUAN Wu-zhou MO Xiao-yang WAN Yong-qi JANG Zhi-gang WU Xiu-shan LI Yong-qing |
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| Institution: | GONG Lin,WANG Yue-qun,YUAN Wu-zhou,MO Xiao-yang,WAN Yong-qi,JANG Zhi-gang,WU Xiu-shanLI Yong-qing*(The Center for Heart Development,Key Lab of MOE for Development Biology and Protein Chemistry,Hunan Normal University,Changsha 410081,China) |
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| Abstract: | To understand the function of the nulp1 protein in early heart development by using Drosophila as a model,here,a fraction of the encoded region in the Drosophila nulp1 gene was obtained by PCR amplification,and then was inserted into pET28a vector to establish the expressing system.Then,the recombinant plasmid was transformed into Rosetta(DE3),and the fusion protein His-nulp1 was induced by IPTG.After purification by Ni2+ metal chelate chromatography,His-nulp1 fusion protein was used to immune the New Zeala... |
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| Keywords: | nulp1 drosophila prokaryotic expression polyclonal antibody |
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