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| 一株高效稻草纤维素降解真菌的筛选与鉴定 | |
| 引用本文： | 熊格生,刘志,吴莎莎,卢向阳,刘如石. 一株高效稻草纤维素降解真菌的筛选与鉴定[J]. 湖南师范大学自然科学学报, 2011, 34(4): 64-68 |
| 作者姓名： | 熊格生 刘志 吴莎莎 卢向阳 刘如石 |
| 作者单位： | 1. 湖南农业大学科学技术师范学院,中国长沙,410128 2. 湖南农业大学生物科学技术学院,中国长沙,410128 3. 湖南师范大学生命科学学院,中国长沙,410081 |
| 基金项目： | 国家自然科学基金资助项目(31072141); 长沙市科技计划资助项目(K0802157-21); 湖南省高等学校科学研究资助项目(10B060); 湖南省科技人才计划项目-省科技计划博士后专项资助项目(2010-2011) |
| 摘 要： | 采用刚果红羧甲基纤维素钠培养基法、滤纸条降解法和胞外酶活性测定法从长白山原始森林的腐烂枯木和落叶腐质土中筛选一株高产纤维素酶的真菌,结合菌落形态学和分子生物学方法鉴定该菌株为产黄青霉,命名为CBS-1.滤纸崩解实验说明在24h该菌对滤纸就具有明显的崩解能力,6d后滤纸的失重率达到24.1％；以稻草粉为底物发酵120 h... |
| 关 键 词： | 水稻秸秆 筛选 纤维索酶 降解微生物 |
Screening and Identification of One Rice Straw-Cellulose Degrading Fungus |
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| XIONG Ge-sheng,LIU Zhi,WU Sha-sha,LU Xiang-yang,LIU Ru-shi. Screening and Identification of One Rice Straw-Cellulose Degrading Fungus[J]. Journal of Natural Science of Hunan Normal University, 2011, 34(4): 64-68 | |
| Authors: | XIONG Ge-sheng LIU Zhi WU Sha-sha LU Xiang-yang LIU Ru-shi |
| Affiliation: | XIONG Ge-sheng1a,LIU Zhi1b,WU Sha-sha1b,LU Xiang-yang1b,LIU Ru-shi2*(1.a.Normal College of Science and Technology,b.College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China,2.College of Life Sciences,Hunan Normal University,Changsha 410081,China) |
| Abstract: | One fungi with high efficient cellulose degrading ability was isolated from the decomposed dead wood and defoliation soil of Changbai mountain primary forest,using hydrolysis spot diameter measurement method of Congo red and CMC-Na,degradation analysis method of filter paper sheet and measurement method of enzymatic activity of cellulase.The strain was identified as Penicillium Chrysogenum with conventional morphology method as well as modern molecular biology method,named CBS-1.Filter paper sheet degradati... |
| Keywords: | rice straw screening cellulase degrading microorganism |
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