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三种抗氧化酶在小麦抗干旱逆境中的作用初探
引用本文:魏炜,赵欣平,吕辉,刘克武,喻东.三种抗氧化酶在小麦抗干旱逆境中的作用初探[J].四川大学学报(自然科学版),2003,40(6):1172-1175.
作者姓名:魏炜  赵欣平  吕辉  刘克武  喻东
作者单位:四川大学生命科学院,成都,610064
摘    要:在模拟干旱条件下连续监测了小麦叶片中的3种抗氧化酶:超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活力变化.发现在干旱胁迫下,此3种酶的活力均会显著升高,但随胁迫天数的不同,其升高幅度会有所差异。同时测定了经过干旱胁迫后恢复培养的小麦叶片中酶活力的大小,发现仅经过中度诱导胁迫的恢复组酶活力有所下降,其它组均继续升高.作者由此认为:干旱胁迫可诱导小麦叶片中的抗氧化酶活力的升高,这些酶有可能协同作用共同抵抗干旱胁迫造成的氧化伤害,但这种诱导作用并非是无限的,重度胁迫可能对植物造成不可逆的伤害.经同工酶电泳检测发现这种诱导作用仅仅导致酶表达量的增加,并没有产生新的同工酶类型。

关 键 词:小麦  抗氧化酶  超氧化物歧化酶(SOD)  过氧化氢酶(CAT)  过氧化物酶(POD)  干旱
文章编号:0490-6756(2003)06-1172-04

The Study of The Function of Three Antioxidant Enzymes in Wheat Leaf Under Drought Stress
WEI Wei,ZHAO Xin-ping,LU Hui,LIU Ke-wu,YU Dong.The Study of The Function of Three Antioxidant Enzymes in Wheat Leaf Under Drought Stress[J].Journal of Sichuan University (Natural Science Edition),2003,40(6):1172-1175.
Authors:WEI Wei  ZHAO Xin-ping  LU Hui  LIU Ke-wu  YU Dong
Abstract:The chang tendency of three antioxidant enzymes activity of wheat leaf under drought stress were surveyed . They are superoxide dismatase (SOD) , peroxidase (POD) and catalase (CAT) . The activities of three enzymes raised notably under drought stress , but the raising ranges were different with the different drought days . These enzymes activities which have resumed after lived under drought stress was also surveyed .Only the resume group's activity which had lived under two day's drought stress had a drop , but the others continued raising . It was showed that drought stress can induce POD , SOD , CAT's activities of wheat leaf to raise , and make them resist the oxidative damage cooperating with each other under drought stress . But this induction isn't no restriction . The heavy stress can make a inreversible damage . From the isoenzyme electrophoresis check test ,it was found that this induction didn't inducted some new isoenzyme types but increased the enzymes' expression quantity .
Keywords:wheat  antioxidant enzymes  superoxide dismatase (SOD)  peroxidase (POD)  catalase (CAT)  drought stress
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