| 浅谈改良CTAB法提取豌豆基因组DNA |
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| 引用本文： | 邹兴华,廖昌伟. 浅谈改良CTAB法提取豌豆基因组DNA[J]. 科技情报开发与经济, 2010, 20(13): 166-167 |
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| 作者姓名： | 邹兴华 廖昌伟 |
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| 作者单位： | 1. 重庆市彭水苗族土家族自治县保家镇农业技术推广服务站,重庆,409607
2. 重庆市彭水苗族土家族自治县保家镇东流完全小学校,重庆,409607 |
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| 摘 要： | 通过改良CTAB法从豌豆（Pisum sativum）嫩叶中提取了基因组DNA,并进行了紫外分光光度计和琼脂糖凝胶电泳分析,结果表明,得到的DNA一级结构较完整,杂质较少,浓度也较高,可进一步用于PCR（Polymerase chain reaction）和酶切等分子生物学实验。
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| 关 键 词： | CTAB法 豌豆 基因组DNA |
Talking about the Isolation of Genomic DNA from Pea with Improved CTAB Method |
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| ZOU Xing-hua,LIAO Chang-wei. Talking about the Isolation of Genomic DNA from Pea with Improved CTAB Method[J]. Sci-Tech Information Development & Economy, 2010, 20(13): 166-167 |
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| Authors: | ZOU Xing-hua LIAO Chang-wei |
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| Affiliation: | ZOU Xing-hua,LIAO Chang-wei |
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| Abstract: | This paper extracts the genomic DNA of the young leaves of pea（Pisum sativum L.）by using an improved CTAB method,and makes the ultraviolet spectrophotometer and agarose gel electrophoresis.The results show that the obtained DNA,which presents better primary structure,higher purity and concentration,could be further used for molecular experiments,including PCR（polymerase chain reaction）and enzyme digestion. |
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| Keywords: | CTAB method pea（Pisum sativum L.） genomic DNA |
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