| L-瓜氨酸与牛血清白蛋白相互作用的研究 |
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| 引用本文： | 屈冉,张骁,张鹏. L-瓜氨酸与牛血清白蛋白相互作用的研究[J]. 北京化工大学学报(自然科学版), 2012, 39(1): 77-82 |
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| 作者姓名： | 屈冉 张骁 张鹏 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029 |
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| 摘 要： | 采用荧光猝灭法以及紫外吸收差谱法研究了L-瓜氨酸与牛血清白蛋白在不同pH值下的相互作用机制，初步了解到L-瓜氨酸对牛血清白蛋白有猝灭作用，其猝灭机制为静态猝灭。利用Stern-Volmer方程求解出的各种参数表明pH值对两者的结合能力影响较大。同步荧光分析发现L-瓜氨酸的存在没有改变牛血清白蛋白的分子构象，依据Forster非辐射能量转移理论，确定了给体-受体间的结合距离和能量转移效率。
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| 关 键 词： | L-瓜氨酸 牛血清白蛋白 相互作用 紫外光谱 荧光猝灭 |
| 收稿时间： | 2011-05-12 |
The interaction between L-citrulline and bovine serum albumin |
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| QU Ran ZHANG Xiao ZHANG Peng. The interaction between L-citrulline and bovine serum albumin[J]. Journal of Beijing University of Chemical Technology, 2012, 39(1): 77-82 |
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| Authors: | QU Ran ZHANG Xiao ZHANG Peng |
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| Affiliation: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China |
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| Abstract: | Fluorescence quenching and ultraviolet absorption subtraction have been employed to study the mechanism of interaction between L-citrulline and bovine serum albumin(BSA) at different pH.The preliminary results show that L-citrulline has the function of quenching BSA,and the quenching mechanism is static.Relevant parameters were obtained using the Stern-Volmer equation.The results showed that pH has a large impact on the binding ability of L-citrulline and BSA.Synchronous fluorescence analysis showed that the conformation of BSA was not changed in the presence of L-citrulline.The combination distance and the efficiency of energy transfer in the donor-acceptor pair were obtained using the non-radiative energy transfer theory of Frster. |
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| Keywords: | L-citrulline bovine serum albumin interaction ultraviolet spectrum fluorescence quenching |
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