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木棉叶中多糖含量的测定
引用本文:杜婷婷,范超君,毕和平.木棉叶中多糖含量的测定[J].海南师院学报,2011(3):307-309.
作者姓名:杜婷婷  范超君  毕和平
作者单位:[1]海南师范大学化学与化工学院,海南海口571158 [2]海南师范大学"省部共建药用植物化学"教育部重点实验室,海南海口571158
基金项目:国家大学生创新实验项目(101165804)
摘    要:黎药木棉叶用石油醚除去脂溶性杂质,用水提醇沉法得海南木棉叶的多糖,采用苯酚-硫酸比色法对多糖含量进行了测定.测得木棉叶的多糖质量分数为0.72%,加样平均回收率为101.1%,相对标准偏差RSD为1.76%.

关 键 词:木棉  多糖  紫外分光光度法

Determination of Polysaccharides Content from Leave of Bombax malabaricum DC
DU Tingting,FAN Chaojun,BI Heping.Determination of Polysaccharides Content from Leave of Bombax malabaricum DC[J].Journal of Hainan Normal University(Humanities and Social Sciences),2011(3):307-309.
Authors:DU Tingting  FAN Chaojun  BI Heping
Institution:1. College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158, China 2. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, Hainan Normal University, Haikou 571158, China)
Abstract:Petroleum ether was used to remove fat-soluble impurities, with Water Extracting-Alcohol Precipitating to get the polysaccharide from leave of Bombax malabaricum DC. The content of polysaccharide was determined with phenol - sulfuric acid colorimetry. The mass fraction of polysaccharide from leave of Bombax malabaricum DC was determined as 0.72% with average recovery of 101.1% and the RSD of 1.76%.
Keywords:Bombax malabaricum DC  Polysaccharide  phenol-sulfuric acid colorimetry
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