| 上海地区正常与黄化香樟表型植株的ISSR特征分析 |
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| 引用本文： | 陈香波,张德顺,毕庆泗,傅徽楠.上海地区正常与黄化香樟表型植株的ISSR特征分析[J].南京林业大学学报(自然科学版),2012(1):33-37. |
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| 作者姓名： | 陈香波 张德顺 毕庆泗 傅徽楠 |
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| 作者单位： | 上海市园林科学研究所;同济大学建筑与城市规划学院;上海市绿化管理指导站 |
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| 基金项目： | 上海市自然科学基金项目(05ZR14108);上海市绿化与市容管理局重点项目(GZB060502) |
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| 摘 要： | 采用ISSR分子标记技术,对上海地区60株不同生长表型(黄化、正常)香樟进行了分子差异遗传分析。使用7条引物PCR扩增,共获得63条清晰的条带,其中61条为多态性位点,多态性位点百分率为96.8%。UPGMA分子聚类结果表明:黄化香樟被单独聚为一类,与正常香樟相分离;两种表现型出现明显的遗传分化,初步证实香樟黄化发生具备一定的遗传基础。
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| 关 键 词： | 香樟 黄化 ISSR 遗传差异 |
Genetic difference between normal and leave chlorosis Cinnamomum camphora detected by ISSR analysis |
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| CHEN Xiangbo,ZHANG Deshun,BI Qingsi,FU Huinan.Genetic difference between normal and leave chlorosis Cinnamomum camphora detected by ISSR analysis[J].Journal of Nanjing Forestry University(Natural Sciences ),2012(1):33-37. |
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| Authors: | CHEN Xiangbo ZHANG Deshun BI Qingsi FU Huinan |
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| Institution: | 1.Shanghai Landscape Gardening Reasearch Institute,Shanghai 200232,China;2.College of Architecture & Urban Planning, Tongji University,Shanghai 200092,China;3.Shanghai Garden Administration & Direction Station,Shanghai 200020,China) |
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| Abstract: | By using ISSR markers,genetic variation among 60 normal and leaf chlorosis Cinnamomum camphora trees in Shanghai were detected.A total of 63 legible bands were amplified.Among which,61 bands exhibit polymorphic.The percentage of polymorphic loci was 96.8 %.UPGMA cluster analysis showed that,trees of the two phenotypes could be categorized into different groups,which indicated that genetic differentiation existing between the two phenotypes.Subsequently,the possibility of breeding resistant clone to leaf chlorosis in C.camphora was discussed. |
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| Keywords: | Cinnamomum camphora leaf chlorosis ISSR genetic variation |
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