| Characteristics of TCRαβ+CD4-CD8- thymocytes in fetal thymic organ culture |
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| 作者姓名： | XIAO Shiyun LI Yan QIAN Xiaoping WANG Yuedan CHEN Weifeng |
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| 作者单位： | Department of Immunology, Peking University Health Science Center, Beijing 100083, China,Department of Immunology, Peking University Health Science Center, Beijing 100083, China,Department of Immunology, Peking University Health Science Center, Beijing 100083, China,Department of Immunology, Peking University Health Science Center, Beijing 100083, China,Department of Immunology, Peking University Health Science Center, Beijing 100083, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (Grant No. 39730410) and the Major State Basic Research Development Program of China |
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| 摘 要： | TCRαβ+CD4-CD8- (TCR+ DN) thymocytes at different developmental periods, i.e. after either 9 or 18 days of culture in the fetal thymic organ culture (FTOC) system, were characterized in the properties of phenotype, proliferation, differentiation and apoptosis. The results showed that anti-CD3 mAb significantly promoted proliferation of TCRαβ+ DN cells generated after 18 days of culture in FTOC, whereas the cells generated after 9 days of culture responded to anti-CD3 mAb by proliferation weakly. IL-7 efficiently induced TCRαβ+ DN cells at day 9 of FTOC to differentiate into TCRαβ+CD4+/CD8+ SP cells without detectable transitional stage of TCRαβ+CD4+CD8+ (DP) cells. In contrast, fewer TCRαβ+ DN cells generated after 18 days of FTOC were induced to differentiate into SP cells. The thymic stromal cell line MTEC5 cells synergized with IL-7 to promote the differentiation of TCRαβ+ DN cells. In addition, TCRαβ+ DN cells were shown to be less susceptible to apoptosis compared with the other major thymocyte subsets. Taken together, these data have provided insight into the characteristics of TCRαβ+ DN thymocytes.
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| 关 键 词： | FTOC TCRαβ+CD4-8- thymocyte |
Characteristics of TCRαβ+CD4-CD8- thymocytes in fetal thymic organ culture |
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| XIAO Shiyun,LI Yan,QIAN Xiaoping,WANG Yuedan,CHEN Weifeng.Characteristics of TCRαβ+CD4-CD8- thymocytes in fetal thymic organ culture[J].Progress in Natural Science,2003,13(6):419-423. |
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| Authors: | XIAO Shiyun LI Yan QIAN Xiaoping WANG Yuedan CHEN Weifeng |
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| Institution: | Department of Immunology, Peking University Health Science Center, Beijing 100083, China |
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| Abstract: | TCRαβ+CD4-CD8- (TCR+ DN) thymocytes at different developmental periods, i.e. after either 9 or 18 days of culture in the fetal thymic organ culture (FTOC) system, were characterized in the properties of phenotype, proliferation, differentiation and apoptosis. The results showed that anti-CD3 mAb significantly promoted proliferation of TCRαβ+ DN cells generated after 18 days of culture in FTOC, whereas the cells generated after 9 days of culture responded to anti-CD3 mAb by proliferation weakly. IL-7 efficiently induced TCRαβ+ DN cells at day 9 of FTOC to differentiate into TCRαβ+CD4+/CD8+ SP cells without detectable transitional stage of TCRαβ+CD4+CD8+ (DP) cells. In contrast, fewer TCRαβ+ DN cells generated after 18 days of FTOC were induced to differentiate into SP cells. The thymic stromal cell line MTEC5 cells synergized with IL-7 to promote the differentiation of TCRαβ+ DN cells. In addition, TCRαβ+ DN cells were shown to be less susceptible to apoptosis compared with the other major thymocyte subsets. Taken together, these data have provided insight into the characteristics of TCRαβ+ DN thymocytes. |
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| Keywords: | FTOC |
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