Hormone content of mouse pancreatic islets subjected to different in vivo and in vitro functional demands |
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Authors: | S. Pääbo G. Lundqvist B. Petersson A. Andersson |
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Affiliation: | (1) Department of Medical Cell Biology, University of Uppsala, Box 571, S-75123 Uppsala, (Sweden);(2) Department of Clinical Chemistry, University Hospital, Uppsala, (Sweden) |
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Abstract: | Summary Mice treated for 4 days with tolbutamide displayed decreased serum glucose values with a concomitant decrease of their islet insulin content. Mouse islets cultured for 1 week at a low (3 mM) or a high (28 mM) glucose concentration contained less insulin than non-cultured islets and islets cultured at a medium (11 mM) glucose concentration. All groups of cultured islets contained more glucagon than non-cultured islets. The somatostatin content of high- and medium-glucose cultured islets was higher than that of freshly isolated islets.The skilled technical assistance of Astrid Nordin, Ewa Forsbeck and Eva Törnelius is gratefully acknowledged. Financial support was received from the Swedish Medical Research Council (12X-109; 12X-2297), the Nordic Insulin Fund and the Swedish Diabetes Association. |
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