| Expression of aquaporin-1 in SMMC-7221 liver carcinoma cells promotes cell migration |
| |
| 作者姓名： | LI Yongming FENG Xuechao YANG Hong MA Tonghui |
| |
| 作者单位： | Membrane Channel Research Laboratory, Northeast Normal University,Changchun 130024, China |
| |
| 基金项目： | We wish to thank Dr. Peter Haggie （University of California, San Francisco, USA） for providing the EYFP-H148Q-V163S expression plasmid. This work was supported by the National Natural Science Fund for Distinguished Young Scholars （Grant No. 30325011）, National Natural Science Foundation of China （Grant No. 30470405）, Distinguished Young Scholars Fund of Jilin Province （Grant No. 20030112）, and Excellent Young Teachers Program of Ministry of Education of China. |
| |
| 摘 要： | Aquaporins (AQPs) are membrane water channels that play pivotal roles in physiological and pathophysi- ological processes in diverse mammalian organs1―3]. Recent studies indicated a novel role of AQPs in cell migration. Mice lacking AQP1, the endothelia…
|
| 关 键 词： | 细胞迁移 瘤转移 细胞容积 肝肿瘤 SMMC-7221 |
| 收稿时间： | 2006-05-20 |
| 修稿时间： | 2006-05-202006-07-20 |
Expression of aquaporin-1 in SMMC-7221 liver carcinoma cells promotes cell migration |
| |
| LI Yongming FENG Xuechao YANG Hong MA Tonghui.Expression of aquaporin-1 in SMMC-7221 liver carcinoma cells promotes cell migration[J].Chinese Science Bulletin,2006,51(20):2466-2471. |
| |
| Authors: | Yongming Li Xuechao Feng Hong Yang Tonghui Ma |
| |
| Institution: | (1) Membrane Channel Research Laboratory, Northeast Normal University, Changchun, 130024, China |
| |
| Abstract: | Migration of tumor cells is a crucial step in tumor invasion and metastasis. Here we provide evidence that aquaporin expression is involved in tumor cell migration. RT-PCR, immunofluorescence and Western blot analysis demonstrated the AQP1 protein expression on the plasma membrane of SMMC-7221 human hepatoma cells. SMMC-7221 cell clones with high (SMMC-7221hPf) and low (SMMC-7221lPf) water permeability were identified by functional assays with corresponding high and low AQP1 expression. Cell migration rate was remarka- bly higher in SMMC-7221hPf cells than SMMC-7221l Pf cells, assessed by Boyden chamber and wound healing assays, whereas cell growth and adhesion were not different. Adenovirus-mediated AQP1 ex- pression in SMMC-7221lPf cells increased their water permeability and migration rate. These results pro- vide the first evidence that aquaporin-mediated membrane water permeability enhances tumor cell migration and may be associated with tumor invasion and metastasis. |
| |
| Keywords: | aquaporin cell migration tumor metastasis cell volume regulation |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
