一种中药复方对小鼠脾脏淋巴细胞PD-1/PD-L1表达的影响

为了探讨中药复方对小鼠脾脏淋巴细胞PD-1/PD-L1的表达情况,以及CD3~+CD4~+和CD3~+CD8~+T淋巴细胞比例的影响,采用传统熬制中药的方法得到中药复方提取液,按低、中、高3个浓度对小鼠灌胃给药(1次/d),2周后,采用脑脊髓脱臼法处死小鼠,利用Real-time PCR和流式细胞仪检测脾脏PD-1/PD-L1的表达情况以及淋巴细胞亚型的变化。结果表明,随着中药提取液浓度的增加,脾脏淋巴细胞PD-1的表达显著降低,而PD-L1的表达无显著变化;CD3~+CD4~+型和CD3~+CD8~+型T淋巴细胞的比例均显著增加。所研制的中药复方能抑制淋巴细胞PD-1的表达,激活免疫辅助细胞和效应细胞,有望应用于抗肿瘤的免疫治疗。

Effect of a kind of Chinese herbal compound on the expression of PD-1/PD-L1 in spleen lymphocytes of mice

In order to investigate the effect on the expression of PD-1/PD-L1 and the proportion of CD3⁺CD4⁺, CD3⁺CD8⁺ T lymphocyte in spleen lymphocytes of mice treated with Chinese herbal compound, the extract of Chinese herbal compound is obtained by traditional boiling method. The mice are given intragastric administration once a day with extract of Chinese herbal compound at low, medium and high concentrations. The mice are sacrificed by neck dislocation at the end of experiment time and the spleen is freshly isolated from mice. The expression of PD-1/PD-L1 and the changes of lymphocyte subtypes are detected by Real-time PCR and flow cytometry. The results show that the expression of PD-1 on splenic lymphocytes decreases significantly and the ratio of CD3⁺CD⁴+ and CD3⁺CD8⁺ T lymphocytes increases significantly with the increase of the concentration of Chinese herbal compound, but the expression of PD-L1 does not change significantly. These results suggest that the Chinese herbal compound can be used in anti-tumor immunotherapy mediated by inhibiting the expression of PD-1 in lymphocytes, activating immune helper cells and effector cells.