大球盖菇黄酮类化合物提取及抑菌性研究

为了探讨大球盖菇黄酮类化合物提取工艺及其抑菌效果,通过正交试验确定了大球盖菇黄酮类化合物的优化提取工艺条件;采用大球盖菇黄酮类化合物对大肠杆菌、青霉菌、啤酒酵母进行抑菌试验.结果表明,优化后的提取工艺为:乙醇体积分数95%,物料比为1∶90,温度60℃,时间120 min,提取率为7.94‰.40 g/L的大球盖菇黄酮类化合物溶液对大肠杆菌和青霉菌有抑制作用;20 g/L和10 g/L的溶液只对大肠杆菌有抑制作用,对青霉菌没有抑制作用;大球盖菇黄酮类化合物对啤酒酵母没有抑制作用;对大肠杆菌的抑制作用要强于对青霉菌的抑制作用.

EXTRACTION OF FLAVONOIDS FROM STROPHARIA RUGOSO-ANNULATA FARLOW AND THE ANTIBACTERIAL ACTIVITY

The flavonoids were extracted from Stropharia rugoso-annulata Farlow and the antibacterial activity on E.coli,Penicillium and S.cerevisiae was studied in this paper.The extraction conditions of flavonoids were optimized by orthographic experiment.The extraction rate could get to 7.94‰ under the optimum extraction conditions including ethanol concentration of 95%,material ratio of 1∶90,60℃,and 120min.The growth of E.coli and Penicillium could be inhibited by 40g/L of the flavonoids from Stropharia rugoso-annulata Farlow.Only E.coli could be inhibited by 20g/L and 10g/L of the flavonoids.The flavonoids showed no obvious inhibition effect on S.cerevisiae.The results indicated that the flavonoids from Stropharia rugoso-annulata Farlow have stronger inhibition effects on E.coli than Penicillium.